Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, 0317 Oslo, Norway.
Genome Biol. 2009 Feb 10;10(2):R13. doi: 10.1186/gb-2009-10-2-r13.
Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (muChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. muChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.
全基因组范围内的组蛋白修饰和转录因子结合的位置分析依赖于染色质免疫沉淀（ChIP）检测。然而，这些检测方法耗时且需要大量的细胞，这限制了它们在许多有趣的细胞类型分析中的应用。我们在此报告了一种快速的微 ChIP（muChIP）检测方法，可对 1000 个细胞进行检测，并与微阵列相结合，从而对组蛋白修饰进行全基因组范围的检测。muChIP-chip 可靠地再现了大规模检测获得的数据：H3K9ac 和 H3K9m3 富集图谱是保守的，并且揭示了无核小体区域。
