Mohammed Javeed Shaikh, Wang Yong, Harvat Tricia A, Oberholzer Jose, Eddington David T
Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA.
Lab Chip. 2009 Jan 7;9(1):97-106. doi: 10.1039/b809590f. Epub 2008 Oct 21.
A microfluidic device to perfuse pancreatic islets while simultaneously characterizing their functionality through fluorescence imaging of the mitochondrial membrane potential and intracellular calcium (Ca(2+)) in addition to enzyme linked immunosorbent assay (ELISA) quantification of secreted insulin was developed and characterized. This multimodal characterization of islet function will facilitate rapid assessment of tissue quality immediately following isolation from donor pancreas and allow more informed transplantation decisions to be made which may improve transplantation outcomes. The microfluidic perfusion chamber allows flow rates of up to 1 mL min(-1), without any noticeable perturbation or shear of islets. This multimodal quantification was done on both mouse and human islets. The ability of this simple microfluidic device to detect subtle variations in islet responses in different functional assays performed in short time-periods demonstrates that the microfluidic perfusion chamber device can be used as a new gold standard to perform comprehensive islet analysis and obtain a more meaningful predictive value for islet functionality prior to transplantation into recipients, which is currently difficult to predict using a single functional assay.
开发并表征了一种微流控装置，该装置可灌注胰岛，同时通过线粒体膜电位和细胞内钙（Ca(2+)）的荧光成像以及酶联免疫吸附测定（ELISA）对分泌胰岛素进行定量来表征胰岛功能。这种对胰岛功能的多模态表征将有助于在从供体胰腺分离后立即快速评估组织质量，并使移植决策更明智，从而可能改善移植结果。微流控灌注室允许流速高达1 mL min(-1)，而不会对胰岛造成任何明显的扰动或剪切。这种多模态定量在小鼠和人类胰岛上均已完成。这种简单的微流控装置能够在短时间内进行的不同功能测定中检测到胰岛反应的细微变化，这表明微流控灌注室装置可作为一种新的金标准，用于在移植到受体之前对胰岛进行全面分析并获得更有意义的胰岛功能预测值，而目前使用单一功能测定很难进行预测。
