微流控装置中长期培养胰岛细胞中气泡的形成和积聚的系统预防。
Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device.
机构信息
Department of Transplant/Surgery, University of Illinois at Chicago, 840 South Wood Street, Clinical Sciences Building, Suite 402, Chicago, IL 60612, USA.
出版信息
Biomed Microdevices. 2012 Apr;14(2):419-26. doi: 10.1007/s10544-011-9618-3.
Abstract
Reliable long-term cell culture in microfluidic system is limited by air bubble formation and accumulation. In this study, we developed a bubble removal system capable of both trapping and discharging air bubbles in a consistent and reliable manner. Combined with PDMS (Polydimethylsiloxane) hydrophilic surface treatment and vacuum filling, a microfluidic perifusion system equipped with the bubble trap was successfully applied for long-term culture of mouse pancreatic islets with no bubble formation and no flow interruption. In addition to demonstrating normal cell viability and islet morphology, post-cultured islets exhibited normal insulin secretion kinetics, intracellular calcium signaling, and changes in mitochondrial potentials in response to glucose challenge. This design could be easily adapted by other microfluidic systems due to its simple design, ease of fabrication, and portability.
摘要
在微流控系统中进行可靠的长期细胞培养受到气泡形成和积累的限制。在这项研究中，我们开发了一种能够以一致且可靠的方式捕获和排出气泡的气泡去除系统。结合 PDMS（聚二甲基硅氧烷）亲水表面处理和真空填充，成功地将配备有气泡捕集器的微流控灌注系统应用于小鼠胰岛的长期培养，没有气泡形成，也没有流动中断。除了证明正常的细胞活力和胰岛形态外，培养后的胰岛还表现出正常的胰岛素分泌动力学、细胞内钙信号和对葡萄糖刺激的线粒体电势变化。由于其简单的设计、易于制造和便携性，这种设计可以很容易地适应其他微流控系统。
相似文献
1
Systematic prevention of bubble formation and accumulation for long-term culture of pancreatic islet cells in microfluidic device.微流控装置中长期培养胰岛细胞中气泡的形成和积聚的系统预防。
Biomed Microdevices. 2012 Apr;14(2):419-26. doi: 10.1007/s10544-011-9618-3.
2
Microfluidic perifusion and imaging device for multi-parametric islet function assessment.用于多参数胰岛功能评估的微流控灌注和成像装置。
Biomed Microdevices. 2010 Jun;12(3):409-17. doi: 10.1007/s10544-010-9398-1.
3
Prevention of air bubble formation in a microfluidic perfusion cell culture system using a microscale bubble trap.使用微尺度气泡捕集器防止微流控灌注细胞培养系统中气泡的形成。
Biomed Microdevices. 2009 Aug;11(4):731-8. doi: 10.1007/s10544-009-9286-8.
4
Passively operated microfluidic device for stimulation and secretion sampling of single pancreatic islets.被动式微流控装置用于刺激和分泌采样单个胰岛。
Anal Chem. 2011 Sep 15;83(18):7166-72. doi: 10.1021/ac201598b. Epub 2011 Aug 16.
5
A simple PDMS-based microfluidic channel design that removes bubbles for long-term on-chip culture of mammalian cells.一种基于 PDMS 的简易微流控通道设计，可去除气泡，实现哺乳动物细胞的长期片上培养。
Lab Chip. 2010 Nov 7;10(21):2906-10. doi: 10.1039/c005274d. Epub 2010 Sep 15.
6
Culturing pancreatic islets in microfluidic flow enhances morphology of the associated endothelial cells.在微流控流中培养胰岛会增强相关内皮细胞的形态。
PLoS One. 2011;6(9):e24904. doi: 10.1371/journal.pone.0024904. Epub 2011 Sep 22.
7
A multi-parametric islet perifusion system within a microfluidic perifusion device.微流控灌注装置内的多参数胰岛灌注系统。
J Vis Exp. 2010 Jan 26(35):1649. doi: 10.3791/1649.
8
A microfluidic twin islets-on-chip device for on-line electrophysiological monitoring.一种用于在线电生理监测的微流控双胰岛芯片装置。
Lab Chip. 2025 Mar 25;25(7):1831-1841. doi: 10.1039/d4lc00967c.
9
Eliminating air bubble in microfluidic systems utilizing integrated in-line sloped microstructures.利用集成在线斜微结构消除微流控系统中的气泡。
Biomed Microdevices. 2020 Oct 22;22(4):76. doi: 10.1007/s10544-020-00529-w.
10
Dual microfluidic perifusion networks for concurrent islet perifusion and optical imaging.双微流控灌流网络用于胰岛同时灌流和光学成像。
Biomed Microdevices. 2012 Feb;14(1):7-16. doi: 10.1007/s10544-011-9580-0.
引用本文的文献
1
Islet-on-a-chip for the study of pancreatic β-cell function.用于研究胰腺β细胞功能的芯片胰岛
In Vitro Model. 2021 Dec 2;1(1):41-57. doi: 10.1007/s44164-021-00005-6. eCollection 2022 Feb.
2
Introducing CELLBLOKS: a novel organ-on-a-chip platform allowing a plug-and-play approach towards building organotypic models.介绍CELLBLOKS：一种新型的芯片上器官平台，允许采用即插即用的方法构建器官型模型。
In Vitro Model. 2022 Jul 4;1(6):423-435. doi: 10.1007/s44164-022-00027-8. eCollection 2022 Dec.
3
Engineered tools to study endocrine dysfunction of pancreas.用于研究胰腺内分泌功能障碍的工程工具。
Biophys Rev (Melville). 2024 Oct 22;5(4):041303. doi: 10.1063/5.0220396. eCollection 2024 Dec.
4
Mesenchymal Stem Cell and Hematopoietic Stem and Progenitor Cell Co-Culture in a Bone-Marrow-on-a-Chip Device toward the Generation and Maintenance of the Hematopoietic Niche.间充质干细胞与造血干祖细胞在芯片上骨髓装置中的共培养，用于造血微环境的生成与维持
Bioengineering (Basel). 2024 Jul 24;11(8):748. doi: 10.3390/bioengineering11080748.
5
Continuous flow delivery system for the perfusion of scaffold-based 3D cultures.用于基于支架的 3D 培养物灌注的连续流动输送系统。
Lab Chip. 2024 Aug 20;24(17):4105-4114. doi: 10.1039/d4lc00480a.
6
Recommendations on fit-for-purpose criteria to establish quality management for microphysiological systems and for monitoring their reproducibility.关于建立微生理系统质量管理和监测其重现性的适用标准的建议。
Stem Cell Reports. 2024 May 14;19(5):604-617. doi: 10.1016/j.stemcr.2024.03.009. Epub 2024 Apr 25.
7
Local Microbubble Removal in Polydimethylsiloxane Microchannel by Balancing Negative and Atmospheric Pressures.通过平衡负压和大气压去除聚二甲基硅氧烷微通道中的局部微泡
Micromachines (Basel). 2023 Dec 23;15(1):37. doi: 10.3390/mi15010037.
8
Micropillar enhanced FRET-CRISPR biosensor for nucleic acid detection.微柱增强的 FRET-CRISPR 生物传感器用于核酸检测。
Lab Chip. 2023 Dec 20;24(1):47-55. doi: 10.1039/d3lc00780d.
9
Microphysiological Models for Mechanistic-Based Prediction of Idiosyncratic DILI.基于机制的药物性肝损伤个体化预测的微生理模型。
Cells. 2023 May 25;12(11):1476. doi: 10.3390/cells12111476.
10
Miniaturizing chemistry and biology using droplets in open systems.利用开放体系中的液滴实现化学和生物学的微型化。
Nat Rev Chem. 2023 Jun;7(6):439-455. doi: 10.1038/s41570-023-00483-0. Epub 2023 Apr 17.
本文引用的文献
1
Application of microfluidic technology to pancreatic islet research: first decade of endeavor.微流控技术在胰岛研究中的应用：首个十年的探索
Bioanalysis. 2010 Oct;2(10):1729-44. doi: 10.4155/bio.10.131.
2
A simple PDMS-based microfluidic channel design that removes bubbles for long-term on-chip culture of mammalian cells.一种基于 PDMS 的简易微流控通道设计，可去除气泡，实现哺乳动物细胞的长期片上培养。
Lab Chip. 2010 Nov 7;10(21):2906-10. doi: 10.1039/c005274d. Epub 2010 Sep 15.
3
Microfluidic perifusion and imaging device for multi-parametric islet function assessment.用于多参数胰岛功能评估的微流控灌注和成像装置。
Biomed Microdevices. 2010 Jun;12(3):409-17. doi: 10.1007/s10544-010-9398-1.
4
Quantitative measurement of zinc secretion from pancreatic islets with high temporal resolution using droplet-based microfluidics.使用基于液滴的微流控技术以高时间分辨率定量测量胰岛的锌分泌。
Anal Chem. 2009 Nov 1;81(21):9086-95. doi: 10.1021/ac9017692.
5
Prevention of air bubble formation in a microfluidic perfusion cell culture system using a microscale bubble trap.使用微尺度气泡捕集器防止微流控灌注细胞培养系统中气泡的形成。
Biomed Microdevices. 2009 Aug;11(4):731-8. doi: 10.1007/s10544-009-9286-8.
6
Microfluidic device for multimodal characterization of pancreatic islets.用于胰岛多模态表征的微流控装置。
Lab Chip. 2009 Jan 7;9(1):97-106. doi: 10.1039/b809590f. Epub 2008 Oct 21.
7
An active bubble trap and debubbler for microfluidic systems.一种用于微流控系统的有源气泡捕集器和除泡器。
Lab Chip. 2008 Oct;8(10):1733-7. doi: 10.1039/b807037g. Epub 2008 Aug 28.
8
Microfluidic approach for rapid interfacial tension measurement.用于快速界面张力测量的微流控方法。
Langmuir. 2008 Oct 7;24(19):11287-92. doi: 10.1021/la801526n. Epub 2008 Sep 12.
9
Analysis of pressure-driven air bubble elimination in a microfluidic device.微流控装置中压力驱动气泡消除的分析
Lab Chip. 2008 Jan;8(1):176-8. doi: 10.1039/b712672g. Epub 2007 Oct 25.
10
Microfluidic bubble logic.微流控气泡逻辑
Science. 2007 Feb 9;315(5813):832-5. doi: 10.1126/science.1136907.
Zotero 插件