Glawdel Tomasz, Almutairi Zeyad, Wang Shuwen, Ren Carolyn
Department of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, Canada.
Lab Chip. 2009 Jan 7;9(1):171-4. doi: 10.1039/b805172k. Epub 2008 Oct 20.
Rhodamine B based fluorescence thermometry is commonly used in microfluidics to measure fluid temperatures in microchannels. Notable absorption of Rhodamine B into PDMS channel walls, however, causes difficulties in obtaining accurate temperature measurements due to a steady increase in the overall fluorescent signal. A simple and effective technique is reported that removes the fluorescent signal from absorbed Rhodamine B dye by means of photobleaching with a high intensity light source before taking images for thermometry analysis. The temperature field at the convergence of hot and cold streams in a Y-channel fabricated in PDMS/glass microfluidic chip is studied to demonstrate the execution of the photobleaching technique.
基于罗丹明B的荧光测温法常用于微流控领域，以测量微通道内的流体温度。然而，罗丹明B显著吸附到聚二甲基硅氧烷（PDMS）通道壁中，由于整体荧光信号持续增加，导致难以获得准确的温度测量结果。本文报道了一种简单有效的技术，即在进行测温分析成像之前，通过用高强度光源进行光漂白，去除吸附的罗丹明B染料产生的荧光信号。研究了在PDMS/玻璃微流控芯片中制造的Y型通道内冷热流体流交汇处的温度场，以证明光漂白技术的实施效果。
