Lin Xiaoyan, Zhao Yuhua, Fu Qinglin, Umashankara M L, Feng Zhihong
Department of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
J Environ Sci (China). 2008;20(12):1494-500. doi: 10.1016/s1001-0742(08)62555-3.
To investigate the influence of bensulfuron-methyl (BSM) on culturable microbial quantities and unculturable microbial community structures, conventional and molecular biological methods were employed in five BSM treated soils with three replications, respectively. The results obtained with traditional culture-dependent methods showed that a low-level of BSM had slight and transient effects on culturable microorganisms; nevertheless, high concentration of BSM resulted in a dramatic decrease in bacterial colony forming units (cfus). The result obtained using denaturing gradient gel electrophoresis (DGGE) revealed that more than 17 bands were observed in low BSM contaminated soil samples and only 10 bands were detected in samples with high BSM contamination. In other words, the diversity of soil community structure is related to the concentration of BSM. Cluster analysis showed that the community structure under low level of contamination was more similar to that of the control, while heavy contaminated amendments were far away from the above group. In a sense, the cooperation of the traditional method and the molecular biological method is more powerful to study the soil microbial information in contaminated ecosystem.
为研究苄嘧磺隆（BSM）对可培养微生物数量和不可培养微生物群落结构的影响，分别采用传统方法和分子生物学方法对5种经BSM处理的土壤进行研究，每种处理设置3个重复。传统的依赖培养方法的结果表明，低水平的BSM对可培养微生物有轻微且短暂的影响；然而，高浓度的BSM导致细菌菌落形成单位（cfus）显著减少。使用变性梯度凝胶电泳（DGGE）获得的结果显示，在低BSM污染的土壤样品中观察到17条以上条带，而在高BSM污染的样品中仅检测到10条带。换句话说，土壤群落结构的多样性与BSM的浓度有关。聚类分析表明，低污染水平下的群落结构与对照更相似，而重度污染的改良剂则与上述组相距较远。从某种意义上说，传统方法与分子生物学方法相结合在研究受污染生态系统中的土壤微生物信息方面更具效力。
