Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, PR, China.
World J Microbiol Biotechnol. 2012 Feb;28(2):649-57. doi: 10.1007/s11274-011-0858-4. Epub 2011 Aug 10.
Wetland degradation makes significant impacts on soil, and bacterial communities in soil are likely to respond to these impacts. The purpose of this study was to investigate the impacts of soil property, soil type and soil depth on bacterial community in different stages of soil degradation in the Zoige Wetland. Microbial biomass carbon was estimated from chloroform fumigation-extraction. Bacterial communities were evaluated by cluster and principal component analysis of DGGE banding patterns and sequencing of partial 16S rDNA PCR amplicons. Experimental results showed that microbial biomass carbon decreased with the soil types (Peat soil > Swamp soil > Meadow soil > Sandy soil) and declined with soil depths (0-20 > 20-40 > 40-60 cm). Bacterial community was affected by soil type more primarily than by soil depth. In addition, the microbial biomass carbon was strongly correlated with soil water content, soil organic carbon and total nitrogen. Sequence analysis of DGGE bands indicated that bacterial phyla of α-Proteobacteria, γ-Proteobacteria, Bacteroidetes, Flavobacterium and Unidentified bacterium predominantly existed in the soil. All these results suggest that specific changes in soil property, soil type and soil depth affected soil bacterial community both quantitatively and qualitatively.
湿地退化对土壤产生重大影响，而土壤中的细菌群落可能会对这些影响做出响应。本研究旨在调查土壤性质、土壤类型和土壤深度对若尔盖湿地土壤退化不同阶段细菌群落的影响。采用氯仿熏蒸提取法估算微生物生物量碳。通过 DGGE 带聚类和主成分分析以及部分 16S rDNA PCR 扩增子测序评估细菌群落。实验结果表明，微生物生物量碳随土壤类型（泥炭土>沼泽土>草地土>沙土）降低，随土壤深度（0-20>20-40>40-60cm）降低。细菌群落受土壤类型的影响大于土壤深度。此外，微生物生物量碳与土壤含水量、土壤有机碳和全氮呈显著正相关。DGGE 条带的序列分析表明，α-变形菌门、γ-变形菌门、拟杆菌门、黄杆菌门和未鉴定细菌门的细菌主要存在于土壤中。所有这些结果表明，土壤性质、土壤类型和土壤深度的特定变化对土壤细菌群落的数量和质量都有影响。
