Reda Mahmoud, Zickri Maha
Theodor Bilharz Research Institute, P.O. Box 30, Imbaba, Giza, Egypt.
J Egypt Soc Parasitol. 2008 Dec;38(3):853-61.
Twenty six male albino rats were divided into a control group (6rats) (G) exposed to Sham operation and two experimental groups (G1 & G2) of 10 rats each. 2/3 partial hepatectomy (PH) was performed in G1 and T4 was administered orally in a dose of 4 mg/kg 10 days prior to PH in G2. Animals were sacrificed 24 hours following PH and Sham operation. Liver sections were immunohistochemically stained for anti-cyclin D1 & anti-VEGF antibodies. Positive reaction was in the nuclei of hepatocytes and in lining of hepatic blood sinusoids respectively. The morphometric determination of the count of cyclin D1 positive nuclei and area % VEGF positive lining of the blood sinusoids was performed in the different groups. Multiple cyclin D1 positive nuclei were detected in liver sections of rats receiving thyroxine prior to PH confirmed by significant increase in the mean count of immuno-positive nuclei as compared to G & G1. VEGF positive immuno-reaction was in the lining of multiple hepatic blood sinusoids on thyroxine therapy proved morphometrically by a significant increase in mean area % in G2 versus G1. The results showed a stimulating effect of thyroxine on liver regeneration following 2/3 partial hepatectomy evidenced by increased Cyclin D and sinusoidal endothelial VEGF expression.
26只雄性白化大鼠被分为一个对照组（6只大鼠）（G组），接受假手术，以及两个实验组（G1组和G2组），每组10只大鼠。G1组进行2/3部分肝切除术（PH），G2组在PH术前10天以4 mg/kg的剂量口服甲状腺素（T4）。在PH和假手术后24小时处死动物。肝组织切片用抗细胞周期蛋白D1和抗血管内皮生长因子（VEGF）抗体进行免疫组织化学染色。阳性反应分别出现在肝细胞的细胞核和肝血窦的内衬中。对不同组进行细胞周期蛋白D1阳性细胞核计数和血窦VEGF阳性内衬面积百分比的形态学测定。与G组和G1组相比，在PH术前接受甲状腺素的大鼠肝组织切片中检测到多个细胞周期蛋白D1阳性细胞核，免疫阳性细胞核的平均计数显著增加，证实了这一点。甲状腺素治疗时，多个肝血窦的内衬出现VEGF阳性免疫反应，通过形态学测定，G2组的平均面积百分比相对于G1组显著增加得到证明。结果表明，甲状腺素对2/3部分肝切除术后的肝脏再生有刺激作用，细胞周期蛋白D和窦状内皮VEGF表达增加证明了这一点。
