Unité de Neuroendocrinologie, INSERM U 159, 2ter, rue d'Alésia, 75014 Paris, France.
J Neuroendocrinol. 1989 Apr 1;1(2):135-9. doi: 10.1111/j.1365-2826.1989.tb00092.x.
Abstract We have studied the responses of growth hormone, prolactin and thyrotrophin to vasoactive intestinal peptide and thyrotrophin-releasing hormone added either together or separately under various in vivo (free-moving, intact animals or rats bearing hypothalamic lesions) or in vitro (perifused anterior pituitary fragments) conditions. Thyrotrophin-releasing hormone or vasoactive intestinal peptide stimulated prolactin release in all cases and the individual effects of both peptides were additive when administered together. Vasoactive intestinal peptide, but not thyrotrophin-releasing hormone, induced a growth hormone response in intact rats. In contrast, both peptides stimulated growth hormone release in lesioned rats as well as in perifused anterior pituitary fragments. In that case, the effect of vasoactive intestinal peptide on growth hormone release was not additive with that of thyrotrophin-releasing hormone, either in vivo or in vitro. Thyrotrophin release was slightly stimulated by vasoactive intestinal peptide, whereas it responded markedly to thyrotrophin-releasing hormone. The effect of thyrotrophin releasing-hormone was not further affected by simultaneous vasoactive intestinal peptide administration. These data suggest that additivity of the effect of second messengers generated as a response to thyrotrophin-releasing hormone and vasoactive intestinal peptide are specific of the target cell type.
摘要 我们研究了在不同的体内（自由活动、完整动物或下丘脑损伤的大鼠）或体外（灌流前垂体片段）条件下，血管活性肠肽和促甲状腺素释放激素同时或分别添加时对生长激素、催乳素和促甲状腺激素的反应。在所有情况下，促甲状腺素释放激素或血管活性肠肽都刺激催乳素释放，当两者同时给药时，其单独作用具有加性。血管活性肠肽而不是促甲状腺素释放激素在完整大鼠中引起生长激素反应。相反，两种肽都刺激损伤大鼠和灌流前垂体片段中的生长激素释放。在这种情况下，血管活性肠肽对生长激素释放的作用与促甲状腺素释放激素在体内或体外均无加性。血管活性肠肽略微刺激促甲状腺素释放，而它对促甲状腺素释放激素的反应明显。促甲状腺素释放激素的作用不受同时给予血管活性肠肽的影响。这些数据表明，作为对促甲状腺素释放激素和血管活性肠肽反应生成的第二信使的作用的加性是特定于靶细胞类型的。
