Rudolf Magnus Institute for Pharmacology, Medical Faculty, University of Utrecht, 3521 GD Utrecht, The Netherlands.
J Neuroendocrinol. 1989 Feb;1(1):47-52. doi: 10.1111/j.1365-2826.1989.tb00075.x.
Abstract Post-translational modifications of vasopressin and oxytocin in pituitary and brain were investigated in view of recent evidence that oxytocin is partly N(alpha)-acetyfated in the bovine pineal gland. Two peptides were isolated from the neurointermediate lobe of the rat pituitary gland and characterized as N(alpha)-acetyl-vasopressin and N(alpha)-acetyl-oxytocin, based on chromatographic and immunological properties as well as the blocked N-terminus. In the neurointermediate pituitary the acetylated forms represented approximately 1% of the vasopressin and oxytocin contents. These two peptides were also detected in some, but not all, investigated brain areas. The highest degree of acetylation was found in the pineal gland. In all regions acetylation of oxytocin was more abundant than that of vasopressin. The data indicate that acetylation of vasopressin and oxytocin generally occurs as a post-translational modification. They support the concept that acetylation may represent a mechanism aimed to control bioactivity of the neurohypophyseal hormones.
摘要 鉴于最近有证据表明牛的松果体内部分催产素被 N(α)-乙酰化，本研究调查了垂体和脑中血管加压素和催产素的翻译后修饰。从大鼠垂体神经中间叶中分离出两种肽，并根据色谱和免疫学特性以及封闭的 N 端，将其鉴定为 N(α)-乙酰血管加压素和 N(α)-乙酰催产素。在神经中间垂体中，乙酰化形式约占血管加压素和催产素含量的 1%。这些两种肽也在一些，但不是所有，研究的脑区中被检测到。乙酰化程度最高的是松果体。在所有区域，催产素的乙酰化程度都高于血管加压素。这些数据表明，血管加压素和催产素的乙酰化通常是一种翻译后修饰。它们支持这样一种概念，即乙酰化可能代表一种旨在控制神经垂体激素生物活性的机制。
