Sutherland J C, Sutherland B M
Department ofPhysiology, University ofCalifornia, Irvine, California 92664, USA.
Biophys J. 1975 May;15(5):435-40. doi: 10.1016/S0006-3495(75)85828-0. Epub 2009 Jan 1.
The action spectrum for photoreactivation by enzymes from human leukocytes and fibroblasts extends from 300 to approximately 600 nm with a maximum near 400 nm. The ability of the human enzymes to utilize light of wavelengths greater than 500 nm suggested that yellow or gold lights conventionally used as safelights for photoreactivation might serve as sources of photoreactivating light for these enzymes. Experiments using lights with a range of spectral outputs confirm that the standard yellow "safe" lights do produce photoreactivation by the human but not the Escherichia coli enzyme.
来自人类白细胞和成纤维细胞的酶进行光复活作用的作用光谱范围为300至约600纳米，最大值接近400纳米。人类酶利用波长大于500纳米光的能力表明，传统上用作光复活安全灯的黄色或金色光可能是这些酶的光复活光源。使用一系列光谱输出的光进行的实验证实，标准的黄色“安全”灯确实能使人的酶产生光复活作用，但不能使大肠杆菌的酶产生光复活作用。
