Jackett P S, Aber V R, Mitchison D A
Am Rev Respir Dis. 1977 Apr;115(4):601-7. doi: 10.1164/arrd.1977.115.4.601.
The relationship between the antibacterial effect of isoniazid and the intracellular concentration of nicotinamide adenine dinucleotide (NAD) was investigated in Mycobacterium tuberculosis strain H37Rv given continuous and pulsed exposures to the drug. Depletion of NAD to a plateau value occurred rapidly during exposure, and recovery after a pulse of isoniazid was also rapid. It seemed unlikely that NAD depletion was the direct cause of the antibacterial activity because (1) insufficient depletion occurred at low isoniazid concentrations; (2) antibacterial activity, but not NAD depletion, was proportional to the product of isoniazid concentration and the exposure period; and (3) NAD depletion was not related to antibacterial activity in cultures of differing physiologic state.
在持续和脉冲式暴露于异烟肼的结核分枝杆菌H37Rv菌株中，研究了异烟肼的抗菌作用与烟酰胺腺嘌呤二核苷酸（NAD）细胞内浓度之间的关系。在暴露期间，NAD迅速耗竭至平稳值，异烟肼脉冲给药后的恢复也很快。NAD耗竭似乎不太可能是抗菌活性的直接原因，因为：（1）在低异烟肼浓度下发生的耗竭不足；（2）抗菌活性而非NAD耗竭与异烟肼浓度和暴露时间的乘积成正比；（3）在不同生理状态的培养物中，NAD耗竭与抗菌活性无关。
