Churchill M, Churchill P C, Schwartz M, Bidani A, McDonald F
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, Michigan.
Kidney Int. 1991 Jul;40(1):13-20. doi: 10.1038/ki.1991.173.
Recently we described methods for optimizing the function of transplanted rat kidneys. In unilaterally nephrectomized recipients, one week after surgery, the left transplanted kidney was identical to the right native kidney with respect to wet weight and the clearances of inulin and para-aminohippuric acid (PAH). The goals of the present experiments were first, to extend the post-surgery period to three weeks (sufficient to allow hypertrophic changes), and second, to study function of transplanted hypertrophied kidneys. Genetically identical Brown Norway rats were used as donor and recipients. Three weeks after transplanting a normal kidney into a unilaterally-nephrectomized recipient, the transplanted kidney had a normal plasma flow and was identical to the contralateral native kidney with respect to wet weight and the clearances of inulin and PAH. Three weeks after transplanting a normal kidney into a bilaterally-nephrectomized recipient, the wet weight, inulin and PAH clearances, and plasma flow of the transplanted kidney were all higher than control, and not significantly different from those observed in unilaterally-nephrectomized control rats. Thus, transplanted and native kidneys exhibited the same degree of compensatory hypertrophy. Hypertrophied donor kidneys (that is, the donor rat had been unilaterally-nephrectomized three weeks previously) remained hypertrophied in bilaterally-nephrectomized recipients, but in unilaterally-nephrectomized recipients, they regressed towards normal (that is, the values of wet weight, inulin and PAH clearances and plasma flow were significantly less than those in rats with only one kidney) while the contralateral native kidney remained normal (values of wet weight and inulin and PAH clearances were not different from control).(ABSTRACT TRUNCATED AT 250 WORDS)
最近我们描述了优化移植大鼠肾脏功能的方法。在单侧肾切除的受体中，术后一周，左移植肾在湿重、菊粉清除率和对氨基马尿酸（PAH）清除率方面与右天然肾相同。本实验的目的，首先是将术后时间延长至三周（足以发生肥大变化），其次是研究移植肥大肾脏的功能。基因相同的棕色挪威大鼠用作供体和受体。将正常肾脏移植到单侧肾切除的受体中三周后，移植肾有正常的血浆流量，在湿重、菊粉清除率和PAH清除率方面与对侧天然肾相同。将正常肾脏移植到双侧肾切除的受体中三周后，移植肾的湿重、菊粉和PAH清除率以及血浆流量均高于对照组，且与单侧肾切除的对照大鼠中观察到的情况无显著差异。因此，移植肾和天然肾表现出相同程度的代偿性肥大。肥大的供体肾脏（即供体大鼠三周前已单侧肾切除）在双侧肾切除的受体中仍保持肥大，但在单侧肾切除的受体中，它们向正常状态回归（即湿重、菊粉和PAH清除率以及血浆流量的值显著低于只有一个肾脏的大鼠），而对侧天然肾保持正常（湿重以及菊粉和PAH清除率的值与对照组无差异）。（摘要截短于250字）
