Min Zhi-Hong, Zhang Ji-Ning, Hua Ke-Qin
The First Maternity and Infant Healthy Institute Affiliated to Fudan University, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Nov;28(11):981-5.
To investigate the regulatory effect of ligustrazine and Radix Astragalus (Chinese drugs for supplementing qi and activating blood circulation) on uterine mucosa stromal cyto-chemotactic factor RANTES and receptor CCR5 expression.
The eutopic and ectopic endometrial stromal cells (EMSC) isolated and purified from 10 patients with ovarian endometrial cyst were selected as the experimental group, while those from 10 patients with benign lesion were selected as the control group. After they were intervened by different factors, including astragalus injection (AI), ligustrazine injection (LI), AI + LI, and Danazol, the expression levels of RANTES and CCR5 in the cells were detected by ELISA and RT-PCR.
RANTES expressions in eutopic and ectopic EMSC were different insignificantly (P > 0.05). The ectopic EMSC RANTES expression was 13.602 +/- 3.358 ng/L and eutopic EMSC RANTES was 12.850 +/- 7.997 ng/L in the positive control group, which was significantly higher than that in the negative control group (both being 0.027 +/- 0.016 ng/L, P <0.05), and the ectopic EMSC expressions after intervened with Chinese drugs were significantly lower than that in the positive control group (P <0.05). CCR5 expression in ectopic EMSC was 0.759 +/- 0.039 ng/L, which was higher than that in eutopic EMSC (0.249 +/- 0.026 ng/L, P <0.05). Ectopic EMSC CCR5 expression was higher in the positive control group than that in the negative control group (0.759 +/- 0.039 ng/L vs 0.478 +/- 0.094 ng/L, P <0.05). Similar situation also was shown between the positive and negative control groups in terms of eutopic EMSC CCR5 expression (0.249 +/- 0.026 ng/L vs 0.131 +/- 0.021 ng/L, P < 0.01), and the expression was significantly lower in all the Chinese drugs treated EMSC groups as compared with that in the positive control group (P < 0.01).
CCR5 expression was higher in ectopic EMSC than that in eutopic EMSC. Ligustrazine and Radix Astragalus could down-regulate the auto-secretion of RANTES and CCR5 in patients with endometriosis.
探讨川芎嗪和黄芪（益气活血中药）对子宫黏膜基质细胞趋化因子调节激活正常T细胞表达和分泌因子（RANTES）及受体CC趋化因子受体5（CCR5）表达的调控作用。
选取10例卵巢子宫内膜样囊肿患者的在位及异位子宫内膜基质细胞（EMSC）作为实验组，选取10例良性病变患者的EMSC作为对照组。分别用黄芪注射液（AI）、川芎嗪注射液（LI）、AI + LI及达那唑进行干预后，采用酶联免疫吸附测定（ELISA）和逆转录-聚合酶链反应（RT-PCR）检测细胞中RANTES和CCR5的表达水平。
在位与异位EMSC中RANTES表达差异无统计学意义（P > 0.05）。阳性对照组中异位EMSC的RANTES表达为13.602±3.358 ng/L，在位EMSC的RANTES表达为12.850±7.997 ng/L，均显著高于阴性对照组（均为0.027±0.016 ng/L，P < 0.05），中药干预后的异位EMSC表达均显著低于阳性对照组（P < 0.05）。异位EMSC中CCR5表达为0.759±0.039 ng/L，高于在位EMSC（0.249±0.026 ng/L，P < 0.05）。阳性对照组异位EMSC的CCR5表达高于阴性对照组（0.759±0.039 ng/L比0.478±0.094 ng/L，P < 0.05）。在位EMSC的CCR5表达在阳性和阴性对照组间也有类似情况（0.249±0.026 ng/L比0.131±0.021 ng/L，P < 0.01），且所有中药处理的EMSC组的表达均显著低于阳性对照组（P < 0.01）。
异位EMSC中CCR5表达高于在位EMSC。川芎嗪和黄芪可下调子宫内膜异位症患者RANTES和CCR5的自分泌。
