Han Jie, Zhou Qiao-ling, Zhang Dan-ying
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Aug;31(8):1113-7.
To assess the inhibitory effects of Neiyi Recipe by comparing adhesive and invasive effects of eutopic and ectopic endometrial tissues from endometriosis women and the endometrium from endometriosis-free women.
The invasive capacity of endometrial stromal cells from endometriosis-free women, eutopic and ectopic endometrial stromal cells from women with endometriosis were compared using Boyden chamber, the chick chorioallantoic membrane (CAM) ectopy transplant, and immunohistochemical EnVision method. The expressions of adhesion and invasion correlated cytokines in the transplanted tissue and effect of Neiyi Recipe on them were observed.
The numbers of invasion stromal cells of eutopic and ectopic endometrial tissues were obviously more than those in the endometriosis-free women (P<0.05). After treatment with Neiyi Recipe and danazol respectively, the number of invasion stromal cells was significantly less in the Neiyi Recipe group than in the danazol group (P<0.05). Compared with endometriosis-free women, the expression of ICAM-1 in the ectopic endometrium tissue, and MMP-9 expressions in the eutopic and ectopic endometrium were significantly higher (all P<0.05) and TIMP-1 expressions obviously lower (P<0.01). In the eutopic endometrium, Neiyi Recipe and danazol serum could significantly lower the expression of MMP-9, and up-regulate expressions of TIMP-1 and ICAM-1 (P<0.01). In the ectopic endometrium, Neiyi Recipe could significantly lower the expression of MMP-9 (P<0.01) and ICAM-1 (P<0.05), and danazol serum could lower the expression of ICAM-1 (P<0.05). Compared with danazol, Neiyi Recipe showed more obvious effect in down-regulating MMP-9 (P<0.05).
High expression of MMP-9 and low expression of TIMP-1 existed in eutopic and ectopic endometrial tissues of endometriosis, which could enhance the ectopic invasion and transplant of endometrial cells. Neiyi Recipe could inhibit the invasion of endometrial stromal cells and the expression of MMP-9.
通过比较子宫内膜异位症患者在位及异位子宫内膜组织与非子宫内膜异位症患者子宫内膜的黏附及侵袭作用，评估内异方的抑制作用。
采用Boyden小室法、鸡胚绒毛尿囊膜（CAM）异位移植法及免疫组织化学EnVision法，比较非子宫内膜异位症患者子宫内膜基质细胞、子宫内膜异位症患者在位及异位子宫内膜基质细胞的侵袭能力。观察移植组织中黏附及侵袭相关细胞因子的表达情况及内异方对其的影响。
在位及异位子宫内膜组织侵袭基质细胞数量明显多于非子宫内膜异位症患者（P<0.05）。分别用内异方和达那唑治疗后，内异方组侵袭基质细胞数量明显少于达那唑组（P<0.05）。与非子宫内膜异位症患者相比，异位子宫内膜组织中ICAM-1表达、在位及异位子宫内膜中MMP-9表达明显升高（均P<0.05），TIMP-1表达明显降低（P<0.01）。在位子宫内膜中，内异方血清和达那唑血清均可明显降低MMP-9表达，上调TIMP-1和ICAM-1表达（P<0.01）。异位子宫内膜中，内异方可明显降低MMP-9表达（P<0.01）和ICAM-1表达（P<0.05），达那唑血清可降低ICAM-1表达（P<0.05）。与达那唑相比，内异方下调MMP-9作用更明显（P<0.05）。
子宫内膜异位症在位及异位子宫内膜组织中存在MMP-9高表达和TIMP-1低表达，可增强子宫内膜细胞的异位侵袭及种植。内异方可抑制子宫内膜基质细胞的侵袭及MMP-9的表达。
