Lai Hong-Shiee, Lin Wen-Hsi, Hsu Wen-Ming, Chen Chiung-Nien, Chang King-Jen, Lee Po-Huang
Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Am Surg. 2009 Jan;75(1):49-54.
Cell-mediated immunity, which includes interferon gamma (IFN-gamma) expression, is activated during the process of liver regeneration; however, the genetic pathway of this activation is still unclear. The present study evaluated variations in the interferon gamma receptor (IFN-gamma R) gene and its mRNA expression during liver regeneration after partial hepatectomy (PH). Male Wistar rats weighing approximately 200 g were subjected to PH (70 or 40%). IFN-gamma R gene expression in the remnant liver was measured by cDNA microarray, and mRNA expression was verified by real-time quantitative reverse transcription-polymerase chain reaction (Q-PCR) preoperatively and at 2, 4, 6, 12, 24, and 72 hours and 7 days postoperatively. The ratio of remnant liver weight to body weight increased markedly after 70 per cent PH and more gradually after 40 per cent PH. It reached near 90 per cent of the preoperative level at 72 hours after PH in both groups. The scanned spots of the genomic survey on the cDNA microarray chips were uneven and increased irregularly in number and density after PH. IFN-gamma R gene expression increased markedly in a single peak pattern, up to more than double the preoperative level, at 6 hours after 70 per cent PH. The curve in the 40 per cent PH group was flat and peaked at only 1.6 times the preoperative level. The variations in IFN-gamma R-related mRNA expression were verified by Q-PCR. Elevations in IFN-gamma R gene and mRNA expression were shown during the early stage of liver regeneration after PH. The genetic pathway of IFN-gamma/IFN-gamma R expression is activated during liver regeneration.
细胞介导的免疫，包括γ干扰素（IFN-γ）表达，在肝脏再生过程中被激活；然而，这种激活的遗传途径仍不清楚。本研究评估了部分肝切除（PH）后肝脏再生过程中γ干扰素受体（IFN-γR）基因及其mRNA表达的变化。对体重约200g的雄性Wistar大鼠进行PH（70%或40%）。术前以及术后2、4、6、12、24、72小时和7天，通过cDNA微阵列测量残余肝脏中IFN-γR基因表达，并通过实时定量逆转录-聚合酶链反应（Q-PCR）验证mRNA表达。70%肝切除术后，残余肝脏重量与体重之比显著增加，40%肝切除术后增加较为缓慢。两组在肝切除术后72小时时，该比值均达到术前水平的近90%。cDNA微阵列芯片上基因组检测的扫描点不均匀，肝切除术后数量和密度不规则增加。在70%肝切除术后6小时，IFN-γR基因表达以单峰模式显著增加，高达术前水平的两倍以上。40%肝切除组的曲线较为平缓，仅在术前水平的1.6倍时达到峰值。通过Q-PCR验证了IFN-γR相关mRNA表达的变化。肝切除术后肝脏再生早期显示IFN-γR基因和mRNA表达升高。在肝脏再生过程中，IFN-γ/IFN-γR表达的遗传途径被激活。
