Garrel D R, Gaudreau P, Zhang L M, Reeves I, Brazeau P
Burn Center, Hotel-Dieu Hospital, Montreal, Quebec, Canada.
J Surg Res. 1991 Oct;51(4):297-302. doi: 10.1016/0022-4804(91)90111-x.
The effects of chronic administration of growth hormone-releasing factor (GRF) on wound healing were studied in rats. Cutaneous wound strength was measured by tensometry at 5, 10, and 14 days postwounding in rats implanted with a slow-release pellet which contained a compressed mixture of a fatty acid and [desamino Tyr1, D-Ala2, Ala15]hGRF(1-29)NH2 or the fatty acid alone. There was a significant increase in wound tensile strength in GRF-treated rats compared to controls at each measurement: Day 5, 130 +/- 12 vs 97 +/- 14 g; Day 10, 402 +/- 18 vs 280 +/- 11 g; Day 14, 830 +/- 17 vs 614 +/- 14 g (P less than 0.01 for each value). Granulation tissue obtained from subcutaneously implanted polyvinyl alcohol sponges encased in silicone tubing was also studied. The amount of collagen deposited in the granulation tissue was estimated by measuring the hydroxyproline (Hyp) content of sponges retrieved 5, 10, and 14 days postinsertion from GRF-treated and control rats. Hyp content (nmole/mg sponge) was similar in both treated and control animals at each measurement: Day 5, 1.7 +/- 0.2 vs 2.2 +/- 0.2; Day 10, 31.9 +/- 4.1 vs 26.7 + 0.4; and Day 14, 41.6 +/- 7.3 vs 38.5 +/- 4.4. Hyp/proline, Hyp/glycine, and glycine/total amino acid ratios, evaluated after 10 days, were also similar in both groups. Collagen from the granulation tissue of sponges retrieved after 14 days from treated and control rats was studied by electron microscopy (magnifications, 7,100 and 22,720).(ABSTRACT TRUNCATED AT 250 WORDS)
在大鼠中研究了长期给予生长激素释放因子（GRF）对伤口愈合的影响。在植入含有脂肪酸和[脱氨基酪氨酸1，D-丙氨酸2，丙氨酸15]hGRF(1-29)NH2的压缩混合物或仅含脂肪酸的缓释微丸的大鼠中，于伤后5、10和14天通过张力测定法测量皮肤伤口强度。与对照组相比，在每次测量时，GRF处理组大鼠的伤口抗张强度均显著增加：第5天，130±12 vs 97±14克；第10天，402±18 vs 280±11克；第14天，830±17 vs 614±14克（每个值P均小于0.01）。还研究了从皮下植入的包裹在硅胶管中的聚乙烯醇海绵获得的肉芽组织。通过测量从GRF处理组和对照组大鼠植入后5、10和14天取出的海绵中羟脯氨酸（Hyp）含量，来估计肉芽组织中沉积的胶原蛋白量。在每次测量时，处理组和对照组动物的Hyp含量（纳摩尔/毫克海绵）相似：第5天，1.7±0.2 vs 2.2±0.2；第10天，31.9±4.1 vs 26.7 + 0.4；第14天，41.6±7.3 vs 38.5±4.4。10天后评估的Hyp/脯氨酸、Hyp/甘氨酸和甘氨酸/总氨基酸比值在两组中也相似。通过电子显微镜（放大倍数为7100和22720）研究了从处理组和对照组大鼠14天后取出的海绵肉芽组织中的胶原蛋白。（摘要截短至250字）
