用于测定大鼠胸膜巨噬细胞产生过氧化氢的荧光法与比色法的比较。

Comparison of a fluorimetric and a colorimetric method for the determination of hydrogen peroxide production by rat pleural macrophages.

作者信息

Von Rein C, Hirschelmann R

机构信息

Sektion Pharmazie, Martin-Luther Universität, Postrach, Halle, Germany.

出版信息

J Pharmacol Methods. 1991 Aug;26(1):61-5. doi: 10.1016/0160-5402(91)90054-9.

DOI:10.1016/0160-5402(91)90054-9

PMID:1921409

Abstract

A fluorimetric and a colorimetric method for the determination of hydrogen peroxide (H2O2) production by isolated cells were compared. Despite a higher sensitivity of the fluorimetric assay, using homovanillic acid (HVA) as reagent, a significantly lower H2O2 production by rat pleural macrophages was measured in comparison to the colorimetric phenol red method. A negative influence of HVA on H2O2 production was detected in the colorimetric assay. These results suggest that the fluorimetric assay with HVA is unsuitable for determining H2O2 formation by isolated cells.

摘要

比较了两种用于测定分离细胞产生过氧化氢（H₂O₂）的方法，即荧光法和比色法。尽管以高香草酸（HVA）作为试剂的荧光测定法具有更高的灵敏度，但与比色法酚红法相比，测得大鼠胸膜巨噬细胞产生的H₂O₂显著更低。在比色测定中检测到HVA对H₂O₂产生有负面影响。这些结果表明，用HVA的荧光测定法不适用于测定分离细胞形成H₂O₂的情况。