Lee S-J, Lim K-T
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, 300 Yongbong-Dong, Gwangju, 500-757, South Korea.
Inflamm Res. 2009 Apr;58(4):184-91. doi: 10.1007/s00011-008-8118-2.
This study was carried out to investigate the anti-inflammatory potentials of 24 kDa glycoprotein isolated from Zanthoxylum piperitum DC fruit (ZPDC glycoprotein) in lipopolysaccharide (LPS)-stimulated murine macrophage cell line (RAW 264.7 cells).
RAW 264.7 cells were treated with ZPDC glycoprotein (50-200 microg/ml) in presence of LPS (2 microg/ml). The changes of the levels of inflammation-related factors were determined by using Western blot, EMSA, and RT-PCR.
ZPDC glycoprotein has inhibitory effects on the phosphorylation of p38 mitogen-activated protein (MAP) kinase and c-Jun N-terminal kinase (JNK), on the DNA binding activity of activator protein-1 (AP-1), and on the expression of c-Jun and c-Fos in LPS-stimulated RAW 264.7 cells. Interestingly, the DNA binding activity of AP-1 was attenuated by treatment with inhibitors of p38 MAP kinase and JNK. In addition, ZPDC glycoprotein (200 microg/ml) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) and proteins (iNOS, COX-2, and MMP-9) in LPS- stimulated RAW 264.7 cells.
The present study demonstrates that ZPDC glycoprotein is a natural anti-oxidant and one of the modulators of pro-inflammatory signal transduction pathways in RAW 264.7 cells.
本研究旨在探讨从花椒果实中分离得到的24 kDa糖蛋白（ZPDC糖蛋白）对脂多糖（LPS）刺激的小鼠巨噬细胞系（RAW 264.7细胞）的抗炎潜力。
在存在LPS（2 μg/ml）的情况下，用ZPDC糖蛋白（50 - 200 μg/ml）处理RAW 264.7细胞。通过蛋白质免疫印迹法、电泳迁移率变动分析和逆转录聚合酶链反应来测定炎症相关因子水平的变化。
ZPDC糖蛋白对LPS刺激的RAW 264.7细胞中p38丝裂原活化蛋白（MAP）激酶和c-Jun氨基末端激酶（JNK）的磷酸化、活化蛋白-1（AP-1）的DNA结合活性以及c-Jun和c-Fos的表达具有抑制作用。有趣的是，用p38 MAP激酶和JNK抑制剂处理可减弱AP-1的DNA结合活性。此外，ZPDC糖蛋白（200 μg/ml）不仅减少了超氧阴离子、过氧化氢和一氧化氮的产生，还抑制了LPS刺激的RAW 264.7细胞中促炎细胞因子（IL-1β、IL-6和TNF-α）以及蛋白质（诱导型一氧化氮合酶、环氧化酶-2和基质金属蛋白酶-9）的表达。
本研究表明ZPDC糖蛋白是一种天然抗氧化剂，也是RAW 264.7细胞中促炎信号转导途径的调节剂之一。
