Pereira Eduardo F, SouzaDe Jurandir Rodrigues
Laboratório de Química Analítica e Ambiental, Universidade de Brasília, Brasília, DF 70919-970, Brazil.
Biol Trace Elem Res. 2009 Sep;130(3):210-7. doi: 10.1007/s12011-009-8332-y. Epub 2009 Feb 13.
A classical cold vapor atomic absorption spectrometric (CVAAS) method for the determination of total mercury in blood has been previously adapted for application to samples of human hair. The reaction vessel specified in the original adaptation was, however, large and difficult to use with small hair samples. In the present study, the reaction vessel has been modified and reduced in size and the protocols have been optimized in order to provide an analytical method that is more efficient, less time-consuming, and gives lower blank values than the original adaptation. The optimized method was validated by multiple, independent, replicated assays of certified reference hair samples, and the mean recovery obtained (98.7%) indicated an efficient performance of the digestion and detection processes. The method was applied to the assay of 144 hair samples derived from populations that had or had not been exposed to mercury from environmental sources. The results from all of the samples analyzed were consistent with those published previously for similar samples.
一种经典的冷蒸气原子吸收光谱法（CVAAS），该方法先前已被改编用于测定血液中的总汞，现在已适用于人类毛发样本。然而，原始改编中指定的反应容器体积较大，对于少量毛发样本难以使用。在本研究中，对反应容器进行了改进并减小了尺寸，同时优化了实验方案，以提供一种比原始改编更高效、耗时更少且空白值更低的分析方法。通过对经过认证的参考毛发样本进行多次独立重复测定，验证了优化后的方法，所获得的平均回收率（98.7%）表明消化和检测过程具有高效性能。该方法应用于分析来自曾暴露或未暴露于环境汞源人群的144份毛发样本。所有分析样本的结果与先前发表的类似样本结果一致。
