用于肿瘤生理微环境研究的人宫颈癌异种移植模型。
Human cervical carcinoma xenograft models for studies of the physiological microenvironment of tumors.
作者信息
Ellingsen Christine, Natvig Ingrid, Gaustad Jon-Vidar, Gulliksrud Kristine, Egeland Tormod A M, Rofstad Einar K
机构信息
Group of Radiation Biology and Tumor Physiology, Department of Radiation Biology, Institute for Cancer Research, Norwegian Radium Hospital, Ullernchausséen 70, 0310 Oslo, Norway.
出版信息
J Cancer Res Clin Oncol. 2009 Sep;135(9):1177-84. doi: 10.1007/s00432-009-0558-8. Epub 2009 Feb 13.
OBJECTIVE
To establish and characterize experimental tumor models of advanced squamous cell carcinoma of the uterine cervix.
METHODS
Permanent cell lines (CK-160 and TS-415) were established from pelvic lymph node metastases of two cervical carcinoma patients. Xenografted tumors were initiated by inoculating 5 x 10(5) cells into the gastrocnemius muscle of BALB/c nu/nu mice. The tumors were characterized with respect to histological appearance, fraction of necrotic tissue (NF), pimonidazole hypoxic fraction (HF(Pim)), interstitial fluid pressure (IFP), extracellular pH (pH(e)), metastatic propensity, and radiation sensitivity.
RESULTS
The xenografted tumors reflected the donor patients' tumors in histological appearance, metastatic propensity, and radiation sensitivity and showed significant intertumor heterogeneity in growth rate, NF, HF(Pim), IFP, and pH(e).
CONCLUSIONS
CK-160 and TS-415 xenografts possess properties making them relevant models for studies of the physiological microenvironment of cervical carcinoma and its influence on metastatic dissemination and response to treatment.
目的
建立并表征晚期子宫颈鳞状细胞癌的实验性肿瘤模型。
方法
从两名宫颈癌患者的盆腔淋巴结转移灶中建立永久细胞系（CK - 160和TS - 415）。通过将5×10⁵个细胞接种到BALB/c nu/nu小鼠的腓肠肌中引发异种移植肿瘤。对肿瘤的组织学外观、坏死组织比例（NF）、匹莫硝唑低氧分数（HF(Pim)）、间质液压力（IFP）、细胞外pH值（pH(e)）、转移倾向和放射敏感性进行表征。
结果
异种移植肿瘤在组织学外观、转移倾向和放射敏感性方面反映了供体患者的肿瘤，并在生长速率、NF、HF(Pim)、IFP和pH(e)方面表现出显著的肿瘤间异质性。
结论
CK - 160和TS - 415异种移植瘤具有使其成为研究宫颈癌生理微环境及其对转移扩散和治疗反应影响的相关模型的特性。
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