在体外生物合成非天然肠球菌素和沃拉普霉素聚酮化合物。
In vitro biosynthesis of unnatural enterocin and wailupemycin polyketides.
机构信息
Scripps Institution of Oceanography and Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, California 92093-0204, USA.
出版信息
J Nat Prod. 2009 Mar 27;72(3):469-72. doi: 10.1021/np800598t.
Abstract
Nature has evolved finely tuned strategies to synthesize rare and complex natural products such as the enterocin family of polyketides from the marine bacterium Streptomyces maritimus. Herein we report the directed ex vivo multienzyme syntheses of 24 unnatural 5-deoxyenterocin and wailupemycin F and G analogues, 18 of which are new. We have generated molecular diversity by priming the enterocin biosynthesis enzymes with unnatural substrates and have illustrated further the uniqueness of this type II polyketide synthase by way of exploiting its unusual starter unit biosynthesis pathways.
摘要
大自然进化出了精细的策略来合成罕见而复杂的天然产物，如来自海洋细菌海洋链霉菌的肠菌素家族聚酮化合物。在此，我们报告了定向离体多酶合成 24 种非天然 5-脱氧肠菌素和 wailupemycin F 和 G 类似物，其中 18 种是新的。我们通过用非天然底物启动肠菌素生物合成酶来产生分子多样性，并通过利用其不寻常的起始单元生物合成途径进一步说明了这种类型 II 聚酮合酶的独特性。
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