University of Edinburgh, Department of Medicine, Western General Hospital, Edinburgh EH4 2XU, UK.
J Neuroendocrinol. 1990 Dec 1;2(6):911-6. doi: 10.1111/j.1365-2826.1990.tb00659.x.
Abstract Corticosteroids exert effects on the hippocampus by binding to intracellular glucocorticoid and/or mineralocorticoid receptors, but the relative importance of each receptor type in mediating corticosteroid effects is poorly understood. There is an extensive serotoninergic (5-HT) innervation of the hippocampus which interacts with corticosteroid-sensitive cells. We have investigated the effect of intracerebroventricular 5,7-dihydroxytryptamine lesions of 5-HT neurons on glucocorticoid and mineralocorticoid receptor messenger ribonucleic acid (mRNA) expression in the rat hippocampus using in situ hybridization histochemistry. In controls, glucocorticoid receptor mRNA was highly expressed in dentate gyrus granule cell neurons, and in pyramidal cells of CA1 and CA2, but levels in CAS and CA4 were significantly lower. 5,7-dihydroxytryptamine-lesioned animals showed significantly less glucocorticoid receptor mRNA in the dentate gyrus (76% decrease), CA1 (42% decrease) and CA2 (52% decrease; all P<0.05 compared with controls). Mineralocorticoid receptor mRNA was expressed at a similar level in all hippocampal subregions in control rats. 5,7-dihydroxytryptamine lesioning led to a significant decrease in mineralocorticoid receptor mRNA expression in CA3 (56% fall) and CA4 (45% fall; both P<0.05), but not in the other subregions. Thus the 5-HT innervation regulates hippocampal corticosteroid receptor mRNA expression.
摘要 皮质甾类通过与细胞内的糖皮质激素和/或盐皮质激素受体结合，对海马体产生作用，但每种受体类型在介导皮质甾类效应中的相对重要性尚不清楚。海马体有广泛的 5-羟色胺能（5-HT）神经支配，与皮质甾类敏感细胞相互作用。我们用原位杂交组织化学法研究了脑室注射 5,7-二羟色胺（5-HT）神经元破坏剂对大鼠海马体中糖皮质激素和盐皮质激素受体信使核糖核酸（mRNA）表达的影响。在对照组中，糖皮质激素受体 mRNA 在齿状回颗粒细胞神经元和 CA1 和 CA2 的锥体细胞中高度表达，但 CA3 和 CA4 中的水平显著较低。5,7-二羟色胺损伤动物的齿状回（减少 76%）、CA1（减少 42%）和 CA2（减少 52%；与对照组相比，所有这些均 P<0.05）中的糖皮质激素受体 mRNA 明显减少。在对照组大鼠的所有海马体亚区中，盐皮质激素受体 mRNA 表达水平相似。5,7-二羟色胺损伤导致 CA3（减少 56%）和 CA4（减少 45%；两者均 P<0.05）中盐皮质激素受体 mRNA 表达显著减少，但其他亚区则没有。因此，5-HT 神经支配调节海马体皮质甾类受体 mRNA 表达。
