Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Neurochem Res. 2010 Nov;35(11):1761-70. doi: 10.1007/s11064-010-0240-1. Epub 2010 Aug 8.
This study aimed to compare the effects of repeated restraint stress alone and the combination with clomipramine treatment on parameters of oxidative stress in cerebral cortex, striatum and hippocampus of male rats. Animals were divided into control and repeated restraint stress, and subdivided into treated or not with clomipramine. After 40 days of stress and 27 days of clomipramine treatment with 30 mg/kg, the repeated restraint stress alone reduced levels of Na(+), K(+)-ATPase in all tissues studied. The combination of repeated restraint stress and clomipramine increased the lipid peroxidation, free radicals and CAT activity as well as decreased levels of NP-SH in the tissues studied. However, Na(+), K(+)-ATPase level decreased in striatum and cerebral cortex and the SOD activity increased in hippocampus and striatum. Results indicated that clomipramine may have deleterious effects on the central nervous system especially when associated with repeated restraint stress and chronically administered in non therapeutic levels.
本研究旨在比较单独重复束缚应激和与氯米帕明治疗联合对雄性大鼠大脑皮质、纹状体和海马氧化应激参数的影响。动物分为对照组和重复束缚应激组，并进一步分为氯米帕明治疗组和未治疗组。经过 40 天的应激和 27 天 30mg/kg 的氯米帕明治疗后，单独重复束缚应激降低了所有研究组织中 Na(+)、K(+)-ATPase 的水平。重复束缚应激和氯米帕明的联合增加了脂质过氧化、自由基和 CAT 活性，同时降低了研究组织中 NP-SH 的水平。然而，Na(+)、K(+)-ATPase 水平在纹状体和大脑皮质中降低，而 SOD 活性在海马体和纹状体中增加。结果表明，氯米帕明可能对中枢神经系统产生有害影响，特别是在与重复束缚应激联合使用且以非治疗水平长期给药时。
