利用RAPD和ISSR标记分析野生地黄的遗传多样性
[Analysis of genetic diversity of wild Rehmannia glutinosa by using RAPD and ISSR markers].
作者信息
Wang Yan, Li Xian-en, Li Xue-dong, Qi Jian-jun, Sun Peng, Zhou Li-li
机构信息
College of Life Sciences, Capital Normal University, Beijing 100037, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(22):2591-5.
OBJECTIVE
To analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplified polymorphic DNA (RAPD) and inter sample sequence repeat (ISSR) for analysis of R. glutinosa accessions.
METHOD
Two molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions.
RESULT
Average 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average (UPGMA) method.
CONCLUSION
A high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher genetic diversity of R. glutinosa germplasms than RAPD marker.
目的
分析野生地黄的遗传多样性，并评估和比较随机扩增多态性DNA（RAPD）和简单重复序列区间（ISSR）用于地黄种质分析的效果。
方法
采用RAPD和ISSR两种分子标记对55份野生地黄种质进行分析。
结果
RAPD引物和ISSR引物分别平均扩增出16.00条和19.08条带，多态性条带比例分别为89.58%和94.32%；采用非加权组平均法（UPGMA）将55份地黄种质分为7个类群。
结论
野生地黄在DNA水平上表现出较高的遗传多样性，不同产地地黄的遗传多样性系数为0.63 - 0.98，且ISSR标记比RAPD标记能检测到更高的地黄种质遗传多样性。
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