Lin Li, Liu Jian-xun, Zhang Ying, Lin Cheng-ren, Li Li-qun
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(22):2683-7.
A sensitive liquid chromatography-mass spectrometric (LC-MS) method for the quantification of salvianolic acid B in rat plasma was developed and validated. The effect of different combination on the salvianolic acid B were studied to investigate SSTG'pharmacokinetics mechanism.
Different combination methods were used for oral administration. The compounds were extracted by solvent in rat plasma and determined using LC-MS-MS method. The results were analysis by DAS software.
Chromatographic separation was achieved on a reversed-phase SB-C18 column with the mobile phase of acetonitrile-methanolformic acid (0.1%) and step gradient elution resulted. The analytes were detected by using an electrospray negative ionization mass spectrometry in the MRM mode. The method showed excellent linearity over the concentration range 0.5-1000 microg x L(-1) (R2=0.999). The low limit of quantification was 0.5 microg x L(-1). The extract recoveries of analytes were from 30.88% to 38.18%. The precisions, the accuracy and the stability all meet the requirements for all analytes. The HPLC-MS-MS technique provided an excellent method for the quantification of SAB in rat plasma.
The method is simple, sensitive and rapid. The plasma concentration of Rg1 and Re were too low to determine. Ginseng & Yanhusuo and Ginseng & Danshen combination can decrease the AUC value and increase the Vd and CL.
建立并验证一种灵敏的液相色谱 - 质谱（LC - MS）法用于定量大鼠血浆中丹酚酸B的含量。研究不同配伍对丹酚酸B的影响，以探讨参芍汤的药代动力学机制。
采用不同的配伍方法进行口服给药。用溶剂萃取大鼠血浆中的化合物，并用LC - MS - MS法进行测定。结果用DAS软件进行分析。
在反相SB - C18柱上以乙腈 - 甲醇 - 甲酸（0.1%）为流动相并采用梯度洗脱实现了色谱分离。采用电喷雾负离子质谱在多反应监测（MRM）模式下检测分析物。该方法在0.5 - 1000μg·L⁻¹浓度范围内具有良好的线性（R² = 0.999）。定量下限为0.5μg·L⁻¹。分析物的萃取回收率为30.88%至38.18%。精密度、准确度和稳定性均符合所有分析物的要求。HPLC - MS - MS技术为定量大鼠血浆中丹酚酸B提供了一种优良的方法。
该方法简便、灵敏、快速。Rg1和Re的血浆浓度过低无法测定。人参 - 延胡索配伍和人参 - 丹参配伍可降低AUC值，增加Vd和CL。
