Jin Hui, Wang Yan, Yan Chao
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(22):2687-91.
To study the adscription of plasma effective constituents of rat after oral administration of Gegen Qinlian decoction.
This study was performed by using HPLC-UV to identify the chemical constituents of rat plasma samples obtained after oral administration of the whole and individual herbs of Gegen Qinlian decoction. A reversed phase Globalsil C18 column (4.6 mm x 250 mm, 5 microm) was used. The mobile phase was composed of 20 mmol x L(-1) ammonium acetate (adjusted to pH 4. 5 by acetate acid) and acetonitrile with gradient elution. The flow rate was 1.0 mL x min(-1) and the column maintained at room temperature. The detection wavelength was set at 270 nm.
The chromatographic analysis of the rat plasma obtained after oral administration of Gegen Qinlian decoction showed 31 compounds, fifteen of which were the original constituents contained in Gegen Qinlian decoction, thirteen of which were metabolites, while the other three only existed after oral administration of the Decoction. By comparison of the retention times of the unknown peaks with that of standard compounds and, by spiking method with the standard compounds, five peaks were identified as puerarin, baicalin, berberine, jatrorrhizine and palmatine.
This HPLC-UV method is simple and reliable in the analysis of the UV absorbing chemical constituents of rat plasma. The compounds absorbed into blood and its metabolites are the possible effective constituents of Gegen Qinlian decoction because they interact directly within the body. The results obtained in this investigation may provide useful information in the further understanding of the action mechanism of the individual and whole herbs in Gegen Qinlian decoction.
研究大鼠口服葛根芩连汤后血浆有效成分的归属。
采用高效液相色谱 - 紫外检测法（HPLC - UV）对大鼠口服葛根芩连汤全方及单味药后获得的血浆样品中的化学成分进行鉴定。使用反相Globalsil C18柱（4.6 mm×250 mm，5μm）。流动相由20 mmol·L⁻¹醋酸铵（用醋酸调至pH 4.5）和乙腈组成，进行梯度洗脱。流速为1.0 mL·min⁻¹，柱温保持在室温。检测波长设定为270 nm。
大鼠口服葛根芩连汤后血浆的色谱分析显示有31种化合物，其中15种为葛根芩连汤原有的成分，13种为代谢产物，另外3种仅在口服该汤剂后出现。通过将未知峰的保留时间与标准化合物的保留时间进行比较，并采用标准化合物加样法，鉴定出5个峰分别为葛根素、黄芩苷、小檗碱、药根碱和巴马汀。
该HPLC - UV方法在分析大鼠血浆中紫外吸收化学成分方面简单可靠。吸收入血的化合物及其代谢产物可能是葛根芩连汤的有效成分，因为它们在体内直接相互作用。本研究所得结果可为进一步了解葛根芩连汤中单味药及全方的作用机制提供有用信息。
