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[用乳胶免疫法测定尿液中的微分子蛋白质和白蛋白。第一部分。方法描述]

[Latex immunologic methods of determining micro-molecular proteins and albumins in urine. Part I. Description of the method].

作者信息

Hałatek T, Jakubowski M

机构信息

Zakładu Biochemii, Instytutu Medycyny Pracy, Lodzi.

出版信息

Med Pr. 1991;42(1):77-87.

PMID:1921718
Abstract

The most frequent markers of early kidney dysfunction include determination of micromolecular proteins, such as beta 2-microglobulin (beta 2M), retinol-binding protein (RBP) and albumins (Alb), in urine. The determination of those proteins in urine is carried out by means of immunological methods. The latex-immunological methods (LIA), allowing to avoid the use of radioisotopes and relatively cheap one as compared with ready-made proprietary kits, may prove to be particularly useful in Polish laboratories. The LIA method in routine tests of protein content in urine was practically assessed. Reproducibility of the method was verified under the conditions of international quality control.

摘要

早期肾功能障碍最常见的标志物包括测定尿液中的微分子蛋白质,如β2-微球蛋白(β2M)、视黄醇结合蛋白(RBP)和白蛋白(Alb)。尿液中这些蛋白质的测定通过免疫学方法进行。乳胶免疫法(LIA)避免了使用放射性同位素,与现成的专利试剂盒相比相对便宜,在波兰实验室可能特别有用。对LIA法在尿液蛋白质含量常规检测中的应用进行了实际评估。在国际质量控制条件下验证了该方法的重现性。

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