Nastasi A, Mammina C, Villafrate M R, Dicuonzo G, Aiello E, Scaglione G
Department of Hygiene and Microbiology G. D'Alessandro, University of Palermo, Italy.
Microbiologica. 1991 Jul;14(3):219-22.
Three strains of Shigella dysenteriae type 2 were isolated from a small household outbreak which occurred in Palermo, Sicily, during summer 1990. Two isolates were recovered from hospitalized patients and one from an asymptomatic carrier. The infection could not be associated with travel to foreign countries or contact with travellers returned from abroad. Since 1953 S. dysenteriae has been never isolated in Southern Italy. The isolates from dysentery cases were susceptible to antibiotics and carried a plasmid of 120 MDa associated with a small cryptic plasmid; in contrast, the strain isolated from the healthy carrier contained an additional plasmid of approximately 40 MDa, which codified for resistance to ampicillin, streptomycin, sulfamethoxazole and trimethoprim. All strains showed some atypical biochemical properties, but their rRNA-DNA patterns of hybridization were closely similar to that of the reference strains of type 2 and easily distinguishable from those of the other types of non-Shiga bacillus reference strains. Epidemiological isolation features of these strains suggest a possible circulation of this Shigella species in Sicily. Genetic characterization of these strains may be useful for surveillance of infections by this organism.
1990年夏季，在西西里岛巴勒莫发生的一起小规模家庭疫情中，分离出了3株2型痢疾志贺菌。其中两株从住院患者中分离得到，一株从无症状携带者中分离得到。此次感染与出国旅行或接触从国外返回的旅行者无关。自1953年以来，意大利南部从未分离出痢疾志贺菌。痢疾病例分离株对抗生素敏感，携带一个120 MDa的质粒以及一个小的隐蔽质粒；相比之下，从健康携带者中分离出的菌株含有一个额外的约40 MDa的质粒，该质粒编码对氨苄青霉素、链霉素、磺胺甲恶唑和甲氧苄啶的耐药性。所有菌株均表现出一些非典型生化特性，但其rRNA - DNA杂交模式与2型参考菌株的模式非常相似，且易于与其他非志贺菌属参考菌株的模式区分开来。这些菌株的流行病学分离特征表明，这种志贺菌在西西里岛可能存在传播。这些菌株的基因特征可能有助于对该生物体引起的感染进行监测。
