Jelesić Z, Kulauzov M, Kozoderović G
Institut za zastitu zdravlja. Medicinski fakultet, Novi Sad.
Med Pregl. 1998 Jul-Aug;51(7-8):305-9.
Shigella spp is one of the most frequently isolated bacteria causing acute diarrhea with us. Genetics of pathogenicity of Shigella spp. includes chromosomal and plasmid genes. Most virulence factors are coded by invasion plasmid antigen genes residing on a 180-230 MDa plasmid. There is a big problem with multiple resistance of Shigella spp. strains, which is mostly plasmid-borne. Genetic analysis of bacterial cells, that is plasmid profile analysis, is important for investigation of sources and ways of spreading of the infection. All isolates originating from the same clone have identical plasmid profiles, i.e. number and size of plasmids. The aim of the investigation was: comparing the type of resistance to antimicrobical agents found in epidemic and nonepidemic. Shigella strains isolated in 1997, analyzing plasmid profiles of these isolates and confirming their epidemic connection.
Susceptibility to antibiotics was examined by a standard disc-diffusion method. Plasmid profiles of 40 strains (20 from the outbreak and 20 from sporadic cases) were tested using a method of alkaline lysis by Birnboim and Doly followed by electrophoresis in agarose gel.
Shigella strains were resistant to antimicrobial agents which are most commonly used. Epidemic isolates shared the same resistance type, they were resistant to cephalexin, streptomycin and co-trimoxazole. The dominant type of resistance of nonepidemic strains was to ampicillin, streptomycin and co-trimoxazole. Strains isolated during the outbreak had identical plasmid profiles (2 plasmid bands of 55 and 1.5 MDa). Non-epidemic isolates had different plasmid profiles as well as type of resistance.
Strains of Shigella spp. isolated during an outbreak had the same type of resistance and the same plasmid profiles, which indicated their origin from the same clone. The plasmid profile analysis is a reliable and precise method for determination of epidemic connection of Shigella isolates.
志贺氏菌属是在我国引起急性腹泻最常分离出的细菌之一。志贺氏菌属的致病性遗传学包括染色体和质粒基因。大多数毒力因子由位于180 - 230 MDa质粒上的侵袭质粒抗原基因编码。志贺氏菌属菌株多重耐药是一个大问题，其耐药性大多由质粒携带。对细菌细胞进行遗传分析，即质粒图谱分析，对于调查感染源和传播途径很重要。源自同一克隆的所有分离株具有相同的质粒图谱，即质粒的数量和大小。本研究的目的是：比较1997年分离出的流行株和非流行株志贺氏菌对抗菌药物的耐药类型，分析这些分离株的质粒图谱并确定它们的流行关联。
采用标准纸片扩散法检测抗生素敏感性。使用Birnboim和Doly的碱裂解法随后在琼脂糖凝胶中进行电泳，检测40株菌株（20株来自暴发疫情，20株来自散发病例）的质粒图谱。
志贺氏菌菌株对最常用的抗菌药物耐药。流行株具有相同的耐药类型，它们对头孢氨苄、链霉素和复方新诺明耐药。非流行株的主要耐药类型是对氨苄西林、链霉素和复方新诺明耐药。暴发疫情期间分离出的菌株具有相同的质粒图谱（55和1.5 MDa的2条质粒带）。非流行株的质粒图谱以及耐药类型各不相同。
暴发疫情期间分离出的志贺氏菌属菌株具有相同的耐药类型和相同的质粒图谱，这表明它们源自同一克隆。质粒图谱分析是确定志贺氏菌分离株流行关联的可靠且精确的方法。
