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[酵母从静止状态转变为活跃生长过程中的恒浊器培养]

[Turbidostat culture of yeast during transition from a resting state to active growth].

作者信息

Kaliuzhin V A

出版信息

Mikrobiologiia. 1991 Mar-Apr;60(2):298-304.

PMID:1921768
Abstract

The age components of a Saccharomyces cerevisiae 14 culture and the kinetics of its growth were studied afer the quiescent state at the onset active growth. The following factors induced the quiescent state: the cessation of a chemostat flow for 24 h, growth inhibition with 2,4-dinitrophenol (DNP) for 24 h, the storage of a culture growing on agar in a refrigerator during 24 h. The process of transition from the point of growth activation to the maximum rate of growth was then studied in turbidostat. This process took the shortest time in a refrigerated culture as well as in a culture that had been limited with a phosphorus source and in a culture limited with a nitrogen source and grown in chemostat at D = 0.26 h-1. The process was longer in cultures that had been either limited with glucose or inhibited with DNP and longest in a chemostat culture limited with a nitrogen source at D = 0.15 and 0.05 h-1. The rate of initial mitosis phases in the yeast is presumed to exert the greatest effect on the duration of this transition process.

摘要

在酿酒酵母14培养物从静止状态进入活跃生长状态后,研究了其年龄组成及其生长动力学。以下因素可诱导静止状态:在恒化器中停止流动24小时、用2,4-二硝基苯酚(DNP)抑制生长24小时、将在琼脂上生长的培养物在冰箱中保存24小时。然后在恒浊器中研究了从生长激活点到最大生长速率的转变过程。在冷藏培养物、受磷源限制的培养物以及受氮源限制并在D = 0.26 h-1的恒化器中生长的培养物中,这个过程耗时最短。在受葡萄糖限制或受DNP抑制的培养物中,这个过程较长,而在D = 0.15和0.05 h-1的受氮源限制的恒化器培养物中,这个过程最长。据推测,酵母中初始有丝分裂阶段的速率对这个转变过程的持续时间影响最大。

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