Gao Cheng, Liu Xiangzhen, Shi Huaizhang, Xu Shancai, Ji Zhiyong, Wang Chunlei, Wu Pei, Liu Zhen, Zhao Shiguang
Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, No.23, Youzheng Street, Nangang District, Harbin, Heilongjiang, 150001, China.
Auton Neurosci. 2009 May 11;147(1-2):70-4. doi: 10.1016/j.autneu.2009.01.010. Epub 2009 Feb 13.
The objective of the present study was to evaluate the correlation between sympathetic nerve activation and inflammatory response in the acute stage of subarachnoid hemorrhage (SAH) in a canine perforating model. SAH was induced by perforation of the basilar artery with the use of a microcatheter via the femoral artery in 20 mongrel dogs. Hemodynamic parameters and intracranial pressure were recorded, and blood sample for C3a, C5b-9, IL-6, IL-8 and noradrenaline kinetic determination were measured at 0, 5, 15, 30, 60, 120, and 180 min after SAH. Noradrenaline (pg/mL) increased abruptly from 104+/-59 to 2010+/-918 at 5 min after SAH. C3a and C5b-9 reached peak values at 15 min and IL-6 and IL-8 reached peak values at 30 min after SAH, respectively. The peak values of C3a and C5b-9 correlated positively with the peak value of noradrenaline (r=0.743 and r=0.753, respectively). The peak values of IL-6 and IL-8 also correlated positively with the peak values of noradrenaline (r=0.603 and r=0.681, respectively).These results suggest that a pronounced activation of the sympathetic nervous system and the inflammatory response occurs in acute stage of SAH. Significant association between the rate of spillover of norepinephrine to plasma and the plasma levels of inflammatory markers indicates that the two processes, sympathetic activation and immune response are quantitatively linked in early stage after SAH. The exact mechanisms underlying this phenomenon deserved further investigations.
本研究的目的是在犬类穿通模型中评估蛛网膜下腔出血（SAH）急性期交感神经激活与炎症反应之间的相关性。通过经股动脉使用微导管穿刺基底动脉，在20只杂种犬中诱导SAH。记录血流动力学参数和颅内压，并在SAH后0、5、15、30、60、120和180分钟测量用于C3a、C5b - 9、IL - 6、IL - 8和去甲肾上腺素动力学测定的血样。SAH后5分钟，去甲肾上腺素（pg/mL）从104±59急剧增加至2010±918。SAH后15分钟C3a和C5b - 9达到峰值，IL - 6和IL - 8分别在SAH后30分钟达到峰值。C3a和C5b - 9的峰值与去甲肾上腺素的峰值呈正相关（分别为r = 0.743和r = 0.753）。IL - 6和IL - 8的峰值也与去甲肾上腺素的峰值呈正相关（分别为r = 0.603和r = 0.681）。这些结果表明，SAH急性期发生交感神经系统和炎症反应的明显激活。去甲肾上腺素向血浆的溢出率与炎症标志物的血浆水平之间存在显著关联，表明交感激活和免疫反应这两个过程在SAH后的早期阶段在数量上是相关联的。这种现象背后的确切机制值得进一步研究。
