Kekarainen Tuija, Martínez-Guinó Laura, Segalés Joaquim
Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Departament de Sanitat i d'Anatomia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
J Gen Virol. 2009 Mar;90(Pt 3):648-653. doi: 10.1099/vir.0.006841-0.
Torque teno viruses (TTVs) are vertebrate infecting, single-stranded circular DNA viruses. Two genetically distinct TTV genogroups (TTV1 and TTV2) infect swine worldwide with high prevalence. Currently, swine TTVs are considered non-pathogenic, although TTV2 has been linked to post-weaning multisystemic wasting syndrome, a porcine circovirus disease. On the other hand, pig materials are an important source of components used in porcine vaccine manufacturing, human drugs and commercial enzyme products. However, there is little information about the possible existence of extraneous viruses in products containing porcine-derived components. In the present study, 26 commercial swine vaccines, seven human drugs and three enzyme products from porcine origin were tested for the presence of TTV1 and TTV2 genomes by PCR. Four vaccines against Mycoplasma hyopneumoniae were positive for TTV2 by PCR. Three M. hyopneumoniae, one porcine parvovirus and one porcine reproductive and respiratory syndrome virus vaccines were PCR positive for TTV1. One human drug contained TTV1 DNA as well as a trypsin enzyme; a porcine-derived elastase product was positive for both TTV genogroups. These results show that swine TTVs are contaminants not only of swine vaccines but also of human drugs containing porcine components and enzymes for laboratory use.
细小病毒（TTVs）是感染脊椎动物的单链环状DNA病毒。两个基因不同的TTV基因组（TTV1和TTV2）在全球范围内以高流行率感染猪。目前，猪TTV被认为是非致病性的，尽管TTV2与断奶后多系统消瘦综合征（一种猪圆环病毒病）有关。另一方面，猪材料是猪疫苗生产、人类药物和商业酶产品中使用的成分的重要来源。然而，关于含有猪源成分的产品中可能存在外源病毒的信息很少。在本研究中，通过PCR检测了26种商业猪疫苗、7种人类药物和3种猪源酶产品中TTV1和TTV2基因组的存在情况。四种抗猪肺炎支原体疫苗经PCR检测TTV2呈阳性。三种猪肺炎支原体疫苗、一种猪细小病毒疫苗和一种猪繁殖与呼吸综合征病毒疫苗经PCR检测TTV1呈阳性。一种人类药物含有TTV1 DNA以及一种胰蛋白酶；一种猪源弹性蛋白酶产品两种TTV基因组均呈阳性。这些结果表明，猪TTV不仅是猪疫苗的污染物，也是含有猪成分的人类药物和实验室用酶的污染物。
