一种新型脱卤杆菌属细菌参与了广泛的4,5,6,7-四氯苯酞脱氯过程。
A novel Dehalobacter species is involved in extensive 4,5,6,7-tetrachlorophthalide dechlorination.
作者信息
Yoshida Naoko, Ye Lizhen, Baba Daisuke, Katayama Arata
机构信息
EcoTopia Science Institute, Nagoya University, Nagoya 464-8603, Japan.
出版信息
Appl Environ Microbiol. 2009 Apr;75(8):2400-5. doi: 10.1128/AEM.02112-08. Epub 2009 Feb 13.
Abstract
The purpose of this study was the enrichment and phylogenetic identification of bacteria that dechlorinate 4,5,6,7-tetrachlorophthalide (commercially designated "fthalide"), an effective fungicide for rice blast disease. Sequential transfer culture of a paddy soil with lactate and fthalide produced a soil-free enrichment culture (designated the "KFL culture") that dechlorinated fthalide by using hydrogen, which is produced from lactate. Phylogenetic analysis based on 16S rRNA genes revealed the dominance of two novel phylotypes of the genus Dehalobacter (FTH1 and FTH2) in the KFL culture. FTH1 and FTH2 disappeared during culture transfer in medium without fthalide and increased in abundance with the dechlorination of fthalide, indicating their growth dependence on the dechlorination of fthalide. Dehalobacter restrictus TEA is their closest relative, with 97.5% and 97.3% 16S rRNA gene similarities to FTH1 and FTH2, respectively.
摘要
本研究的目的是对能够使4,5,6,7-四氯邻苯二甲酰亚胺（商品名为“稻瘟酞”，一种防治稻瘟病的有效杀菌剂）脱氯的细菌进行富集培养和系统发育鉴定。用乳酸盐和稻瘟酞对稻田土壤进行连续传代培养，得到了一种无土富集培养物（命名为“KFL培养物”），该培养物利用乳酸盐产生的氢气使稻瘟酞脱氯。基于16S rRNA基因的系统发育分析表明，脱卤杆菌属的两个新系统型（FTH1和FTH2）在KFL培养物中占主导地位。在不含稻瘟酞的培养基中进行培养传代时，FTH1和FTH2消失，随着稻瘟酞的脱氯，其丰度增加，这表明它们的生长依赖于稻瘟酞的脱氯。严格脱卤杆菌TEA是它们最接近的亲缘种，与FTH1和FTH2的16S rRNA基因相似性分别为97.5%和97.3%。
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