酿酒酵母产细菌素菌株的开发，该菌株可异源表达并分泌来自粪肠球菌L50的无前导肽肠球菌素L50肽L50A和L50B。
Development of bacteriocinogenic strains of Saccharomyces cerevisiae heterologously expressing and secreting the leaderless enterocin L50 peptides L50A and L50B from Enterococcus faecium L50.
作者信息
Basanta Antonio, Herranz Carmen, Gutiérrez Jorge, Criado Raquel, Hernández Pablo E, Cintas Luis M
机构信息
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
出版信息
Appl Environ Microbiol. 2009 Apr;75(8):2382-92. doi: 10.1128/AEM.01476-08. Epub 2009 Feb 13.
Abstract
A segregationally stable expression and secretion vector for Saccharomyces cerevisiae, named pYABD01, was constructed by cloning the yeast gene region encoding the mating pheromone alpha-factor 1 secretion signal (MFalpha1(s)) into the S. cerevisiae high-copy-number expression vector pYES2. The structural genes of the two leaderless peptides of enterocin L50 (EntL50A and EntL50B) from Enterococcus faecium L50 were cloned, separately (entL50A or entL50B) and together (entL50AB), into pYABD01 under the control of the galactose-inducible promoter P(GAL1). The generation of recombinant S. cerevisiae strains heterologously expressing and secreting biologically active EntL50A and EntL50B demonstrates the suitability of the MFalpha1(s)-containing vector pYABD01 to direct processing and secretion of these antimicrobial peptides through the S. cerevisiae Sec system.
摘要
通过将编码交配信息素α因子1分泌信号（MFalpha1(s)）的酵母基因区域克隆到酿酒酵母高拷贝数表达载体pYES2中，构建了一种用于酿酒酵母的分离稳定表达和分泌载体，命名为pYABD01。来自粪肠球菌L50的肠球菌素L50的两个无信号肽的结构基因（EntL50A和EntL50B）分别（entL50A或entL50B）和一起（entL50AB）在半乳糖诱导型启动子P(GAL1)的控制下克隆到pYABD01中。异源表达和分泌生物活性EntL50A和EntL50B的重组酿酒酵母菌株的产生证明了含MFalpha1(s)的载体pYABD01适用于通过酿酒酵母Sec系统指导这些抗菌肽的加工和分泌。
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