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本文引用的文献

1
Performance and applications of polyclonal antipeptide antibodies specific for the enterococcal bacteriocin enterocin P.针对肠球菌细菌素肠球菌素P的多克隆抗肽抗体的性能及应用
J Agric Food Chem. 2004 Apr 21;52(8):2247-55. doi: 10.1021/jf030551j.
2
Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins.不同来源肠球菌中肠球菌素的筛选及溶血素和万古霉素耐药性检测
Int J Food Microbiol. 2003 Aug 1;84(3):299-318. doi: 10.1016/s0168-1605(02)00425-7.
3
Production of class II bacteriocins by lactic acid bacteria; an example of biological warfare and communication.乳酸菌产生II类细菌素；生物战与交流的一个例子。
Antonie Van Leeuwenhoek. 2002 Aug;81(1-4):639-54. doi: 10.1023/a:1020582211262.
4
Characterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 from Lactobacillus plantarum 423, in Saccharomyces cerevisiae.植物乳杆菌423中IIa类细菌素植物乳杆菌素423在酿酒酵母中的特性鉴定及异源表达
Int J Food Microbiol. 2003 Feb 25;81(1):29-40. doi: 10.1016/s0168-1605(02)00164-2.
5
Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality.产细菌素的乳酸菌在改善食品安全和质量方面的潜力。
Biochimie. 2002 May-Jun;84(5-6):593-604. doi: 10.1016/s0300-9084(02)01457-8.
6
Heterologous expression of bacteriocins using the mesentericin Y105 dedicated transport system by Leuconostoc mesenteroides.嗜柠檬酸明串珠菌利用Mesentericin Y105专用转运系统进行细菌素的异源表达。
Biochimie. 2002 May-Jun;84(5-6):569-76. doi: 10.1016/s0300-9084(02)01413-x.
7
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.在毕赤酵母发酵罐培养物中生产重组蛋白。
Curr Opin Biotechnol. 2002 Aug;13(4):329-32. doi: 10.1016/s0958-1669(02)00330-0.
8
Bacteriocins: safe, natural antimicrobials for food preservation.细菌素：用于食品保鲜的安全天然抗菌剂。
Int J Food Microbiol. 2001 Dec 4;71(1):1-20. doi: 10.1016/s0168-1605(01)00560-8.
9
Incidence of virulence factors and antibiotic resistance among Enterococci isolated from food.从食品中分离出的肠球菌的毒力因子和抗生素耐药性发生率。
Appl Environ Microbiol. 2001 Sep;67(9):4385-9. doi: 10.1128/AEM.67.9.4385-4389.2001.
10
Expression level of heterologous proteins in Pichia pastoris is influenced by flask design.毕赤酵母中外源蛋白的表达水平受摇瓶设计的影响。
Appl Microbiol Biotechnol. 2001 May;55(4):463-5. doi: 10.1007/s002530000479.

从屎肠球菌P13中产生的抗李斯特菌的类片球菌素细菌素肠球菌素P在毕赤酵母中的表达。

Production of enterocin P, an antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, in Pichia pastoris.

作者信息

Gutiérrez Jorge, Criado Raquel, Martín María, Herranz Carmen, Cintas Luis M, Hernández Pablo E

机构信息

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

出版信息

Antimicrob Agents Chemother. 2005 Jul;49(7):3004-8. doi: 10.1128/AAC.49.7.3004-3008.2005.

DOI:10.1128/AAC.49.7.3004-3008.2005
PMID:15980385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168690/
Abstract

The gene encoding mature enterocin P (EntP), an antimicrobial peptide from Enterococcus faecium P13, was cloned into the pPICZalphaA expression vector to generate plasmid pJC31. This plasmid was integrated into the genome of P. pastoris X-33, and EntP was heterologously secreted from the recombinant P. pastoris X-33t1 derivative at a higher production and antagonistic activity than from E. faecium P13.

摘要

编码成熟肠球菌素P（EntP）的基因，一种来自屎肠球菌P13的抗菌肽，被克隆到pPICZalphaA表达载体中以产生质粒pJC31。该质粒整合到巴斯德毕赤酵母X-33的基因组中，并且EntP从重组巴斯德毕赤酵母X-33t1衍生物中异源分泌，其产量和拮抗活性高于屎肠球菌P13。