从屎肠球菌P13中产生的抗李斯特菌的类片球菌素细菌素肠球菌素P在毕赤酵母中的表达。
Production of enterocin P, an antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, in Pichia pastoris.
作者信息
Gutiérrez Jorge, Criado Raquel, Martín María, Herranz Carmen, Cintas Luis M, Hernández Pablo E
机构信息
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
出版信息
Antimicrob Agents Chemother. 2005 Jul;49(7):3004-8. doi: 10.1128/AAC.49.7.3004-3008.2005.
Abstract
The gene encoding mature enterocin P (EntP), an antimicrobial peptide from Enterococcus faecium P13, was cloned into the pPICZalphaA expression vector to generate plasmid pJC31. This plasmid was integrated into the genome of P. pastoris X-33, and EntP was heterologously secreted from the recombinant P. pastoris X-33t1 derivative at a higher production and antagonistic activity than from E. faecium P13.
摘要
编码成熟肠球菌素P（EntP）的基因，一种来自屎肠球菌P13的抗菌肽，被克隆到pPICZalphaA表达载体中以产生质粒pJC31。该质粒整合到巴斯德毕赤酵母X-33的基因组中，并且EntP从重组巴斯德毕赤酵母X-33t1衍生物中异源分泌，其产量和拮抗活性高于屎肠球菌P13。
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