Ziora D, Dworniczak S, Kozielski J
Department of Lung Diseases and Tuberculosis, Medical University of Silesia, Zabrze, Poland.
J Physiol Pharmacol. 2008 Dec;59 Suppl 6:809-17.
The aim of this study was to assess sputum levels of the metalloproteinases MMP-1, MMP-2, MMP-3, TIMP-1, and TIMP-2, as well as MMPs/TIMPs ratios in relation to exhaled NO (eNO) and sputum NOs (nitrates and nitrites) and IL8 obtained from chronic obstructive pulmonery disease (COPD) patients, healthy non-smokers, and healthy smokers. We found higher levels of TIMP-1 (118.9 ng/ml) and TIMP-2 (3.75 ng/ml) in COPD patients than in healthy smokers (17.7 ng/ml, P<0.03; 0.51 ng/ml, P>0.05, respectively) and healthy non-smokers (84.6 ng/ml, P>0.05; 1.61 ng/ml, P>0.05, respectively). We also observed significant positive correlations between concentrations of NOs and MMP-1, MMP-2, MMP-3, and TIMP-2 (r=0.37, P<0.02; r=0.60, P<0.0001; r=0.56, P<0.0004 and r=0.47 P<0.004, respectively) in COPD patients. IL8, MMP-2, MMP-3, and TIMP-2 levels in induced sputum were negatively correlated with airway obstruction, i.e., FEV(1)/FVC (r=-0.61, P<0.00009; r=-0.41, P<0.01; r=-0.38, P<0.02; r=-0.49, P<0.002). Our study points to a potentially pathogenic role of stromelysin-1 (MMP-3) in COPD.
本研究旨在评估慢性阻塞性肺疾病（COPD）患者、健康非吸烟者和健康吸烟者痰液中金属蛋白酶MMP-1、MMP-2、MMP-3、TIMP-1和TIMP-2的水平，以及MMPs/TIMPs比值与呼出一氧化氮（eNO）、痰液一氧化氮（硝酸盐和亚硝酸盐）和白细胞介素8（IL8）的关系。我们发现，COPD患者的TIMP-1（118.9 ng/ml）和TIMP-2（3.75 ng/ml）水平高于健康吸烟者（分别为17.7 ng/ml，P<0.03；0.51 ng/ml，P>0.05）和健康非吸烟者（分别为84.6 ng/ml，P>0.05；1.61 ng/ml，P>0.05）。我们还观察到，COPD患者中，一氧化氮浓度与MMP-1、MMP-2、MMP-3和TIMP-2之间存在显著正相关（r分别为0.37，P<0.02；r为0.60，P<0.0001；r为0.56，P<0.0004；r为0.47，P<0.004）。诱导痰液中的IL8、MMP-2、MMP-3和TIMP-2水平与气道阻塞呈负相关，即与FEV(1)/FVC呈负相关（r分别为-0.61，P<0.00009；r为-0.41，P<0.01；r为-0.38，P<0.02；r为-0.49，P<0.002）。我们的研究表明基质溶解素-1（MMP-3）在COPD中可能具有致病作用。
