香烟烟雾与唾液的协同效应。
Synergistic effects of cigarette smoke and saliva.
作者信息
Nozad-Mojaver Yalda, Mirzaee Maysam, Jafarzadeh Abdullah
机构信息
Department of Oral Medicine, Rafsanjan University of Medical Sciences, Dental school, Rafsanjan, Iran.
出版信息
Med Oral Patol Oral Cir Bucal. 2009 May 1;14(5):E217-21.
OBJECTIVES
The aim of this study was to evaluate the cytotoxic effects of Cigarette Smoke on the human peripheral blood lymphocytes in the presence of stimulated or non-stimulated saliva in an in vitro model.
METHODS AND MATERIALS
Ten healthy volunteers in the age range of 21 to 29 were selected and samples of peripheral blood lymphocytes and saliva (whole and stimulated saliva) collected . Peripheral blood lymphocytes suspensions (PBS) were taken and exposed to 6 different media. Samples were collected from all media at both 20 and 80 minute time points. The survival rates of PBL were then determined at both 20 and 80 minute time points.
RESULTS
The cell survival rates following exposure to cigarette smoke (CS) in the presence of PBS supplemented with whole or stimulated saliva were significantly lower at 80 minutes when compared with the 20 minute rates (P<0.05).
CONCLUSION
Tobacco effects were significantly increased in the presence of saliva especially stimulated saliva.
目的
本研究的目的是在体外模型中，评估香烟烟雾在存在刺激唾液或未刺激唾液的情况下对人外周血淋巴细胞的细胞毒性作用。
方法和材料
选取10名年龄在21至29岁之间的健康志愿者，采集外周血淋巴细胞和唾液样本（全唾液和刺激唾液）。提取外周血淋巴细胞悬液（PBS）并使其暴露于6种不同培养基中。在20分钟和80分钟时间点从所有培养基中采集样本。然后在20分钟和80分钟时间点测定外周血淋巴细胞的存活率。
结果
与20分钟时相比，在添加全唾液或刺激唾液的PBS存在下，暴露于香烟烟雾（CS）80分钟后的细胞存活率显著降低（P<0.05）。
结论
在存在唾液尤其是刺激唾液的情况下，烟草的影响显著增加。
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