Cousin Anouska, Nelson Matthew N
Canola Breeders Western Australia Pty Ltd, Locked Bag 888, Como, WA, Australia.
Plant Cell Rep. 2009 May;28(5):831-5. doi: 10.1007/s00299-009-0677-3. Epub 2009 Feb 15.
Microspore culture is used extensively in several crop species to generate diverse populations of homozygous, doubled haploid lines for breeding and genetic analyses. In our canola (Brassica napus L.) doubled haploid breeding programme we regularly observe conjoined microspore-derived embryos, most commonly twins, joined either at the base of the hypocotyl or along the length of the hypocotyl axis. The aim of this study was to determine if twinned embryos were genetically identical or non-identical in order to gauge their value for breeding and linkage analysis. Microsatellite marker fingerprinting of 12 pairs of twinned embryos produced by microspore culture of heterozygous F(1) lines revealed that pairs of twins were genetically identical. Based on this finding, we recommend breeders and geneticists using microspore culture technology to retain only one embryo from each pair of twinned embryos.
小孢子培养在多种作物中被广泛应用，用于培育用于育种和遗传分析的纯合双单倍体系的多样化群体。在我们的油菜（甘蓝型油菜L.）双单倍体育种计划中，我们经常观察到小孢子衍生的连体胚胎，最常见的是双胞胎，它们在胚轴基部或沿胚轴长度相连。本研究的目的是确定连体胚胎在遗传上是相同还是不同，以便评估它们在育种和连锁分析中的价值。对杂合F(1)系小孢子培养产生的12对连体胚胎进行微卫星标记指纹分析，结果表明双胞胎对在遗传上是相同的。基于这一发现，我们建议使用小孢子培养技术的育种者和遗传学家每对连体胚胎只保留一个胚胎。
