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多样性数组技术标记：油菜（甘蓝型油菜）中的遗传多样性分析和连锁图谱构建。

Diversity array technology markers: genetic diversity analyses and linkage map construction in rapeseed (Brassica napus L.).

机构信息

EH Graham Centre for Agricultural Innovation, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW 2650, Australia.

出版信息

DNA Res. 2012;19(1):51-65. doi: 10.1093/dnares/dsr041. Epub 2011 Dec 22.

DOI:10.1093/dnares/dsr041
PMID:22193366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3276259/
Abstract

We developed Diversity Array Technology (DArT) markers for application in genetic studies of Brassica napus and other Brassica species with A or C genomes. Genomic representation from 107 diverse genotypes of B. napus L. var. oleifera (rapeseed, AACC genomes) and B. rapa (AA genome) was used to develop a DArT array comprising 11 520 clones generated using PstI/BanII and PstI/BstN1 complexity reduction methods. In total, 1547 polymorphic DArT markers of high technical quality were identified and used to assess molecular diversity among 89 accessions of B. napus, B. rapa, B. juncea, and B. carinata collected from different parts of the world. Hierarchical cluster and principal component analyses based on genetic distance matrices identified distinct populations clustering mainly according to their origin/pedigrees. DArT markers were also mapped in a new doubled haploid population comprising 131 lines from a cross between spring rapeseed lines 'Lynx-037DH' and 'Monty-028DH'. Linkage groups were assigned on the basis of previously mapped simple sequence repeat (SSRs), intron polymorphism (IP), and gene-based markers. The map consisted of 437 DArT, 135 SSR, 6 IP, and 6 gene-based markers and spanned 2288 cM. Our results demonstrate that DArT markers are suitable for genetic diversity analysis and linkage map construction in rapeseed.

摘要

我们开发了多样性数组技术（DArT）标记物，用于研究甘蓝型油菜和其他具有 A 或 C 基因组的芸薹属物种的遗传。利用来自 107 个不同甘蓝型油菜（甘蓝型油菜，AACC 基因组）和油菜（AA 基因组）基因型的基因组代表性序列，开发了一个包含 11520 个克隆的 DArT 阵列，这些克隆是使用 PstI/BanII 和 PstI/BstN1 复杂度降低方法生成的。总共鉴定出 1547 个具有高技术质量的多态性 DArT 标记物，用于评估来自世界各地的 89 个甘蓝型油菜、油菜、芥菜和黑芥的 1547 个多态性 DArT 标记物的分子多样性。基于遗传距离矩阵的层次聚类和主成分分析确定了不同的群体聚类，主要根据其起源/血统进行聚类。DArT 标记还在一个新的双单倍体群体中进行了映射，该群体由春油菜品系“Lynx-037DH”和“Monty-028DH”杂交产生的 131 个品系组成。根据先前映射的简单序列重复（SSR）、内含子多态性（IP）和基于基因的标记物，将连锁群分配到基于基因的标记物上。该图谱包含 437 个 DArT、135 个 SSR、6 个 IP 和 6 个基于基因的标记物，覆盖 2288 cM。我们的结果表明，DArT 标记物适合于油菜的遗传多样性分析和连锁图谱构建。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/3b5b9bfc72da/dsr04103a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/73deb1b13474/dsr04101.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/baf446c0a230/dsr04102.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/3b5b9bfc72da/dsr04103a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/73deb1b13474/dsr04101.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/baf446c0a230/dsr04102.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f61/3276259/3b5b9bfc72da/dsr04103a.jpg

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