使用靶标引发和分支滚环扩增技术对微小RNA进行高灵敏度测定。
Highly sensitive determination of microRNA using target-primed and branched rolling-circle amplification.
作者信息
Cheng Yongqiang, Zhang Xian, Li Zhengping, Jiao Xiaoxia, Wang Yucong, Zhang Yali
机构信息
Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, China.
出版信息
Angew Chem Int Ed Engl. 2009;48(18):3268-72. doi: 10.1002/anie.200805665.
One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined.
基于分支滚环扩增（BRCA）反应和荧光定量分析，可以区分微小RNA（miRNA）中的单核苷酸差异。采用该方法，可检测低至10 fM浓度的miRNA，并可测定几纳克总RNA样品中的miRNA。
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