Department of Ophthalmology, Firat University School of Medicine, Elaziğ, Turkey.
Acta Ophthalmol. 2009 Nov;87(8):871-4. doi: 10.1111/j.1755-3768.2008.01368.x.
We aimed to determine the extent of protection provided by Visudyne eyeglass against vascular endothelial growth factor (VEGF) synthesis following photodynamic therapy (PDT).
Three groups with 14 rabbits in each were established. These consisted of a control (dextrose infusion) group, an infusion (verteporfin infusion) group and an irradiation (verteporfin infusion + irradiation) group. One eye in each animal was closed with Visudyne eyeglass and the other by eyelid sutures. The rabbits were exposed to daylight for 30 mins at 2 and 48 hours after the infusion was administered. Half the animals in each group were killed on day 5. The remaining animals were killed on day 10. Levels of VEGF in homogenized retina and choroids were analysed with an ELISA (enzyme-linked immunosorbent assay).
Mean VEGF levels, in pg/mg protein, on days 5 and 10 in the control + glass, control + suture, infusion + glass, infusion + suture, irradiation + glass and irradiation + suture subgroups were, respectively: 1.69 +/- 0.67, 1.91 +/- 0.44; 1.75 +/- 0.69, 1.93 +/- 0.53; 2.30 +/- 0.77, 3.47 +/- 2.02; 1.90 +/- 1.00, 2.93 +/- 0.16; 4.39 +/- 2.74, 13.63 +/- 5.25; 3.38 +/- 1.05, 7.37 +/- 2.12. On day 10, VEGF levels were significantly higher in the infusion and irradiation groups compared with the control group (p < 0.05). There were no statistically significant differences between glass and suture samples on days 5 and 10 in the infusion group, or on day 5 in the irradiation group. However, on day 10, the mean VEGF level in eyes closed with Visudyne eyeglass in the irradiation group was significantly higher than in sutured eyes (p = 0.011).
Visudyne eyeglass offers full protection against VEGF increases caused by verteporfin infusion but is only partially protective in eyes exposed to sensitizing light.
我们旨在确定 Visudyne 眼镜在光动力疗法（PDT）后对血管内皮生长因子（VEGF）合成的保护程度。
建立了三组，每组 14 只兔子。它们分别是对照组（葡萄糖输注）、输注组（维替泊芬输注）和照射组（维替泊芬输注+照射）。每只动物的一只眼睛用 Visudyne 眼镜闭合，另一只用眼睑缝合闭合。在输注后 2 小时和 48 小时，兔子暴露在阳光下 30 分钟。每组一半的动物在第 5 天处死。其余动物在第 10 天处死。用 ELISA（酶联免疫吸附试验）分析匀浆视网膜和脉络膜中的 VEGF 水平。
第 5 天和第 10 天，对照组+玻璃、对照组+缝合、输注+玻璃、输注+缝合、照射+玻璃和照射+缝合亚组的平均 VEGF 水平（pg/mg 蛋白）分别为：1.69±0.67、1.91±0.44；1.75±0.69、1.93±0.53；2.30±0.77、3.47±2.02；1.90±1.00、2.93±0.16；4.39±2.74、13.63±5.25；3.38±1.05、7.37±2.12。第 10 天，与对照组相比，输注和照射组的 VEGF 水平明显升高（p<0.05）。在输注组，第 5 天和第 10 天，玻璃和缝合样本之间无统计学差异；但在照射组，第 5 天，闭合 Visudyne 眼镜的眼睛的平均 VEGF 水平明显高于缝合眼睛（p=0.011）。
Visudyne 眼镜可完全防止维替泊芬输注引起的 VEGF 增加，但在暴露于敏化光的眼睛中仅部分保护。
