Department of Ophthalmology, Firat University School of Medicine, Elazig, Turkey.
Indian J Ophthalmol. 2011 Jan-Feb;59(1):5-8. doi: 10.4103/0301-4738.73705.
To determine the retinal nitric oxide (NO) and malonyldialdehyde (MDA) levels following photodynamic therapy (PDT).
Seven Dutch-belted rabbits received dextrose, while seven others received 2 mg/kg verteporfin infusion over a period of 15 minutes in a dim-lit room. Irradiation to a 1.5 mm diameter intact chorioretinal area in the right eye of verteporfin-infused rabbits, was started 5 minutes after the end of infusion. Three groups were control (dextrose infusion), infusion with verteporfin (left eyes were not irradiated), and irradiation after verteporfin injection (right eyes were irradiated). On the fifth day of the experiment, the eyes were enucleated. The retinas were subsequently frozen and homogenized. Nitrite, a stable end-product of NO and MDA, was measured using the spectrophotometer. Protein concentrations were measured by the Lowry method. Tissue NO and MDA levels were expressed as μmol/gprt and nmol/mgprt, respectively.
The mean retinal NO and MDA levels of the control, infusion, and irradiation groups were 24.67 ± 6.66, 0.11 ± 0.02; 45.90 ± 15.52, 0.21 ± 0.09; and 84.43 ± 14.96 μmol/gprt, 0.58 ± 0.14 nmol/mgprt, respectively. The mean retinal NO levels were significantly elevated in the infusion and irradiation groups compared with the control group ( p :0.004; p :0.001). The mean retinal MDA levels were significantly elevated in the infusion and irradiation groups compared to the control one ( p :0.026; p :0.001). Also the mean retinal NO and MDA levels in the irradiation group were found to be significantly higher than the infusion group ( p :0.018; p :0.018).
Not only PDT, but also verteporfin infusion alone resulted in NO and MDA level increments in the retina, which might be toxic.
为了确定光动力疗法（PDT）后视网膜的一氧化氮（NO）和丙二醛（MDA）水平。
7 只荷兰兔给予葡萄糖，另 7 只给予 2mg/kg 维替泊芬静脉输注，在暗光房间内输注 15 分钟。维替泊芬输注结束后 5 分钟开始对右眼进行 1.5mm 直径完整脉络膜视网膜区域的照射。实验第 5 天眼球被摘除。视网膜随后冷冻并匀浆。使用分光光度计测量亚硝酸盐，其为 NO 的稳定终产物和 MDA。蛋白浓度通过 Lowry 法测量。组织中的 NO 和 MDA 水平分别以 μmol/gprt 和 nmol/mgprt 表示。
对照组、输注组和照射组的视网膜平均 NO 和 MDA 水平分别为 24.67 ± 6.66、0.11 ± 0.02μmol/gprt；45.90 ± 15.52、0.21 ± 0.09μmol/gprt；84.43 ± 14.96μmol/gprt、0.58 ± 0.14nmol/mgprt。与对照组相比，输注组和照射组的视网膜平均 NO 水平显著升高（p：0.004；p：0.001）。与对照组相比，输注组和照射组的视网膜平均 MDA 水平显著升高（p：0.026；p：0.001）。照射组的视网膜 NO 和 MDA 水平也明显高于输注组（p：0.018；p：0.018）。
不仅 PDT，单独的维替泊芬输注也会导致视网膜中 NO 和 MDA 水平升高，这可能是有毒的。
