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鸡卵黄生成素基因结合蛋白是一种亮氨酸拉链转录因子，它能与鸡卵黄生成素II启动子中的一个重要调控元件结合，与大鼠DBP相关。

Chicken vitellogenin gene-binding protein, a leucine zipper transcription factor that binds to an important control element in the chicken vitellogenin II promoter, is related to rat DBP.

作者信息

Iyer S V, Davis D L, Seal S N, Burch J B

机构信息

Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.

出版信息

Mol Cell Biol. 1991 Oct;11(10):4863-75. doi: 10.1128/mcb.11.10.4863-4875.1991.

DOI:10.1128/mcb.11.10.4863-4875.1991
PMID:1922023
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC361455/
Abstract

We screened a chicken liver cDNA expression library with a probe spanning the distal region of the chicken vitellogenin II (VTGII) gene promoter and isolated clones for a transcription factor that we have named VBP (for vitellogenin gene-binding protein). VBP binds to one of the most important positive elements in the VTGII promoter and appears to play a pivotal role in the estrogen-dependent regulation of this gene. The protein sequence of VBP was deduced from a nearly full length cDNA copy and was found to contain a basic/zipper (bZIP) motif. As expected for a bZIP factor, VBP binds to its target DNA site as a dimer. Moreover, VBP is a stable dimer free in solution. A data base search revealed that VBP is related to rat DBP. However, despite the fact that the basic/hinge regions of VBP and DBP differ at only three amino acid positions, the DBP binding site in the rat albumin promoter is a relatively poor binding site for VBP. Thus, the optimal binding sites for VBP and DBP may be distinct. Similarities between the VBP and DBP leucine zippers are largely confined to only four of the seven helical spokes. Nevertheless, these leucine zippers are functionally compatible and appear to define a novel subfamily. In contrast to the bZIP regions, other portions of VBP and DBP are markedly different, as are the expression profiles for these two genes. In particular, expression of the VBP gene commences early in liver ontogeny and is not subject to circadian control.

摘要

我们用跨越鸡卵黄生成素II（VTGII）基因启动子远端区域的探针筛选了鸡肝cDNA表达文库，并分离出了一个转录因子的克隆，我们将其命名为VBP（卵黄生成素基因结合蛋白）。VBP与VTGII启动子中最重要的正调控元件之一结合，似乎在该基因的雌激素依赖性调控中起关键作用。VBP的蛋白质序列是从一个近乎全长的cDNA拷贝推导出来的，发现它含有一个碱性/拉链（bZIP）基序。正如对bZIP因子的预期，VBP以二聚体形式与其靶DNA位点结合。此外，VBP是一种在溶液中游离的稳定二聚体。数据库搜索显示VBP与大鼠DBP相关。然而，尽管VBP和DBP的碱性/铰链区仅在三个氨基酸位置不同，但大鼠白蛋白启动子中的DBP结合位点对VBP来说是一个相对较差的结合位点。因此，VBP和DBP的最佳结合位点可能不同。VBP和DBP亮氨酸拉链之间的相似性主要局限于七个螺旋辐条中的四个。尽管如此，这些亮氨酸拉链在功能上是兼容的，似乎定义了一个新的亚家族。与bZIP区域不同，VBP和DBP的其他部分明显不同，这两个基因的表达谱也是如此。特别是，VBP基因的表达在肝脏个体发育早期开始，不受昼夜节律控制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1200/361455/f15b43adf97f/molcellb00034-0078-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1200/361455/f15b43adf97f/molcellb00034-0078-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1200/361455/496f4df2a181/molcellb00034-0072-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1200/361455/af42d9dd488f/molcellb00034-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1200/361455/07feaa521348/molcellb00034-0076-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1200/361455/f15b43adf97f/molcellb00034-0078-b.jpg

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