Walker P, Germond J E, Brown-Luedi M, Givel F, Wahli W
Nucleic Acids Res. 1984 Nov 26;12(22):8611-26. doi: 10.1093/nar/12.22.8611.
In the liver of oviparous vertebrates vitellogenin gene expression is controlled by estrogen. The nucleotide sequence of the 5' flanking region of the Xenopus laevis vitellogenin genes A1, A2, B1 and B2 has been determined. These sequences have been compared to each other and to the equivalent region of the chicken vitellogenin II and apo-VLDLII genes which are also expressed in the liver in response to estrogen. The homology between the 5' flanking region of the Xenopus genes B1 and B2 is higher than between the corresponding regions of the other closely related genes A1 and A2. Four short blocks of sequence homology which are present at equivalent positions in the vitellogenin genes of both Xenopus laevis and chicken are characterized. A short sequence with two-fold rotational symmetry (GGTCANNNTGACC) was found at similar positions upstream of the five vitellogenin genes and is also present in two copies close to the 5' end of the chicken apo-VLDLII gene. The possible functional significance of this sequence, common to liver estrogen-responsive genes, is discussed.
在卵生脊椎动物的肝脏中，卵黄蛋白原基因的表达受雌激素调控。非洲爪蟾卵黄蛋白原基因A1、A2、B1和B2的5'侧翼区域的核苷酸序列已被确定。这些序列相互之间以及与鸡卵黄蛋白原II和载脂蛋白-VLDLII基因的相应区域进行了比较，鸡的这些基因也在肝脏中受雌激素诱导表达。非洲爪蟾基因B1和B2的5'侧翼区域之间的同源性高于其他亲缘关系较近的基因A1和A2的相应区域之间的同源性。鉴定出了在非洲爪蟾和鸡的卵黄蛋白原基因的等效位置上存在的四个短序列同源块。在五个卵黄蛋白原基因上游的相似位置发现了一个具有双重旋转对称性的短序列（GGTCANNNTGACC），并且在靠近鸡载脂蛋白-VLDLII基因5'端的位置也有两个拷贝。讨论了这个肝脏雌激素反应基因共有的序列可能的功能意义。
