核因子——GATA、VBP和雌激素受体在增强卵黄蛋白原基因转录中的协同作用。
Synergistic effects of nuclear factors--GATA, VBP and ER in potentiating vitellogenin gene transcription.
作者信息
Teo B Y, Tan N S, Lam T J, Ding J L
机构信息
National University of Singapore, Marine Biotechnology Laboratory, Department of Biological Sciences, 10 Kent Ridge Crescent, Singapore, Singapore.
出版信息
FEBS Lett. 1999 Oct 1;459(1):57-63. doi: 10.1016/s0014-5793(99)01212-0.
The Oreochromis aureus vitellogenin (OaVtg) gene contains three imperfect oestrogen response elements (EREs) and GATA and VBP (vitellogenin binding protein) binding sites. An analysis of the promoter indicates that the 5'-flanking region up to position -625 is sufficient to mediate E(2) control. Furthermore, transfection of deletion and mutagenised promoters indicates that both GATA and VBP synergise with ER, and thus contribute to the regulation of the endogenous OaVtg gene. These findings support the notion that the interplay of promoter elements mediates proper hormone-dependent and tissue-specific expression of the OaVtg gene, regardless of non-consensus sequence context of EREs and VBP.
奥利亚罗非鱼卵黄蛋白原（OaVtg）基因包含三个不完全雌激素反应元件（ERE）以及GATA和卵黄蛋白原结合蛋白（VBP）结合位点。对该启动子的分析表明，至-625位的5'侧翼区域足以介导E(2)调控。此外，缺失和诱变启动子的转染表明，GATA和VBP均与雌激素受体协同作用，从而有助于内源性OaVtg基因的调控。这些发现支持这样一种观点，即启动子元件之间的相互作用介导了OaVtg基因适当的激素依赖性和组织特异性表达，而与ERE和VBP的非共有序列背景无关。
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