Wu H, Reynolds A B, Kanner S B, Vines R R, Parsons J T
Department of Microbiology, Health Sciences Center, Charlottesville, Virginia 22908.
Mol Cell Biol. 1991 Oct;11(10):5113-24. doi: 10.1128/mcb.11.10.5113-5124.1991.
Transformation of cells by the src oncogene results in elevated tyrosine phosphorylation of two related proteins, p80 and p85 (p80/85). Immunostaining with specific monoclonal antibodies revealed a striking change of subcellular localization of p80/85 in src-transformed cells. p80/85 colocalizes with F-actin in peripheral extensions of normal cells and rosettes (podosomes) of src-transformed cells. Sequence analysis of cDNA clones encoding p80/85 revealed an amino-terminal domain composed of six copies of a direct tandem repeat, each repeat containing 37 amino acids, a carboxyl-terminal SH3 domain, and an interdomain region composed of a highly charged acidic region and a region rich in proline, serine, and threonine. The multidomain structure of p80/85 and its colocalization with F-actin in normal and src-transformed cells suggest that these proteins may associate with components of the cytoskeleton and contribute to organization of cell structure.
src癌基因导致的细胞转化会使两种相关蛋白p80和p85（p80/85）的酪氨酸磷酸化水平升高。用特异性单克隆抗体进行免疫染色显示，src转化细胞中p80/85的亚细胞定位发生了显著变化。在正常细胞的外周延伸部位以及src转化细胞的玫瑰花结（黏着斑）中，p80/85与F-肌动蛋白共定位。对编码p80/85的cDNA克隆进行序列分析发现，其氨基末端结构域由六个直接串联重复序列组成，每个重复序列包含37个氨基酸，一个羧基末端的SH3结构域，以及一个由高电荷酸性区域和富含脯氨酸、丝氨酸和苏氨酸的区域组成的结构域间区域。p80/85的多结构域结构及其在正常细胞和src转化细胞中与F-肌动蛋白的共定位表明，这些蛋白可能与细胞骨架成分相关，并有助于细胞结构的组织。
