Moriguchi C, Egami C, Kawata Y, Terakawa S, Tsuchimori M, Watanabe O
Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu, Shizuoka 432-8561, Japan.
J Microsc. 2009 Feb;233(2):269-74. doi: 10.1111/j.1365-2818.2009.03117.x.
We have developed a stroboscopic near-field optical microscope for observation of biological specimens and observed glycerinated muscles before and after muscle contraction with the developed system. In the system, the optical field distribution localized near the specimen is recorded as the surface topographic distribution of a photosensitive film surface. Our system is very useful for observing living biological specimens with high resolutions, because it is possible to get stroboscopic image by using a photosensitive film as detecting optical distributions instead of a scanning of probes. We have succeeded in observing inner structures of muscle cells with sub-wavelength resolution and achieved higher contrast than an ordinary optical microscope.
我们开发了一种用于观察生物标本的频闪近场光学显微镜，并用该系统观察了甘油化肌肉收缩前后的情况。在该系统中，位于标本附近的光场分布被记录为感光胶片表面的表面形貌分布。我们的系统对于高分辨率观察活的生物标本非常有用，因为通过使用感光胶片来检测光分布而不是扫描探针就可以获得频闪图像。我们成功地以亚波长分辨率观察到了肌肉细胞的内部结构，并实现了比普通光学显微镜更高的对比度。
