Whitaker B D, Knight J W
Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA, USA.
Reprod Domest Anim. 2010 Oct;45(5):755-9. doi: 10.1111/j.1439-0531.2009.01344.x.
This study was conducted to evaluate the effects of different concentrations of the antioxidant N-acetyl-cysteine (NAC) supplemented to the maturation medium on porcine embryo development. Concentrations of NAC and its synthetic derivative, NAC-amide (NACA) were evaluated for effects on nuclear maturation, fertilization success and embryo development. Concentrations of NAC (0, 0.5, 1.0, 1.5, 2.0, 2.5 and 5.0 mm) were supplemented to maturing oocytes, and embryo development was analysed at 48 and 144 h post-fertilization. There were no differences among cleavage rates for any of the treatment groups. Blastocyst formation for 1.5 mm NAC (56.5 ± 9.2%) was higher (p < 0.05) than all other supplementations. There were no differences in nuclear maturation or fertilization or in cleavage rates when comparing 1.5 mm NAC and 1.5 mm NACA supplementation to the control. Blastocyst formation for 1.5 mm NAC (44.4 ± 4.7%) and 1.5 mm NACA (46.2 ± 3.4%) supplementation were higher (p < 0.05) than the control (32.1 ± 6.2%) oocytes. These results indicate that supplementing 1.5 mm of NAC or NACA to the oocyte maturation medium increased the percentage of viable embryos reaching the blastocyst stage of development.
本研究旨在评估向成熟培养液中添加不同浓度的抗氧化剂N-乙酰半胱氨酸（NAC）对猪胚胎发育的影响。评估了NAC及其合成衍生物NAC-酰胺（NACA）的浓度对核成熟、受精成功率和胚胎发育的影响。向成熟的卵母细胞中添加不同浓度的NAC（0、0.5、1.0、1.5、2.0、2.5和5.0 mM），并在受精后48小时和144小时分析胚胎发育情况。各处理组的卵裂率没有差异。添加1.5 mM NAC时的囊胚形成率（56.5±9 .2%）高于所有其他添加组（p<0.05）。将添加1.5 mM NAC和1.5 mM NACA与对照组进行比较时，核成熟、受精或卵裂率没有差异。添加1.5 mM NAC（44.4±4.7%）和1.5 mM NACA（46.2±3.4%）时的囊胚形成率高于对照组卵母细胞（32.1±6.2%）（p<0.05）。这些结果表明，向卵母细胞成熟培养液中添加1.5 mM的NAC或NACA可提高发育至囊胚阶段的活胚胎百分比。
