Curlevski Nathalie J A, Chambers Susan M, Anderson Ian C, Cairney John W G
Centre for Plant and Food Science, University of Western Sydney, Penrith South DC, NSW, Australia.
FEMS Microbiol Ecol. 2009 Mar;67(3):411-20. doi: 10.1111/j.1574-6941.2008.00637.x.
Assemblages of fungi associated with roots of cooccurring Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) seedlings at a sclerophyll forest site in New South Wales, Australia, were investigated by direct DNA extraction and analysis of rRNA gene internal transcribed spacer (ITS) products by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) analyses. While ordination of the DGGE data suggested that the assemblages did not differ significantly between the two plant taxa, T-RFLP data provided marginal statistical support for the presence of different assemblages. Fungi isolated from roots of both plants were identified by ITS sequence comparisons largely as ascomycetes, several of which had close sequence identity to Helotiales ericoid mycorrhizal (ERM) fungi. One isolate morphotype from E. pulchella had close sequence similarity to ectomycorrhizal fungi in the Cenococcum geophilum complex, and neighbour-joining analysis grouped this strongly with other Australian C. geophilum-like sequences. Distribution of genotypes of an ERM Helotiales ascomycete in root systems of the two plant taxa was also investigated using inter-simple sequence repeat (ISSR)-PCR. Nineteen ISSR genotypes were identified, two of which were present in roots of both plant taxa. The results are discussed in the context of potential mycelial connections between Ericaceae and non-Ericaceae plants.
通过直接DNA提取，并运用变性梯度凝胶电泳（DGGE）和末端限制性片段长度多态性（T-RFLP）分析rRNA基因内转录间隔区（ITS）产物，对澳大利亚新南威尔士州一处硬叶林中共生的美丽澳石南（杜鹃花科）和多花细籽桉（桃金娘科）幼苗根系相关的真菌组合进行了研究。虽然DGGE数据的排序表明这两种植物类群的真菌组合没有显著差异，但T-RFLP数据为不同组合的存在提供了微弱的统计支持。通过ITS序列比较，从两种植物根系中分离出的真菌大多被鉴定为子囊菌，其中几种与柔膜菌目杜鹃类菌根（ERM）真菌具有密切的序列相似性。从美丽澳石南分离出的一种分离物形态型与土生隐球菌复合体中的外生菌根真菌具有密切的序列相似性，邻接法分析将其与其他澳大利亚类土生隐球菌序列紧密聚类在一起。还利用简单重复序列区间（ISSR）-PCR研究了一种ERM柔膜菌目子囊菌在两种植物类群根系中的基因型分布。共鉴定出19种ISSR基因型，其中两种存在于两种植物类群的根系中。本文在杜鹃花科和非杜鹃花科植物之间潜在的菌丝连接背景下讨论了研究结果。
