Wang Ya, Lai Zheng, Li Xi-Xi, Yan Ri-Ming, Zhang Zhi-Bin, Yang Hui-Lin, Zhu Du
Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of life Sciences, Jiangxi Science and Technology Normal University, Nanchang, 330013, China.
Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun, 336000, China.
World J Microbiol Biotechnol. 2016 Feb;32(2):20. doi: 10.1007/s11274-015-1966-3. Epub 2016 Jan 8.
Huperzia serrata has many important medicinal properties with proven pharmacological potential. Some of these properties may be mediated by its endophytic fungi. To test this hypothesis, in the present study, we provided a first insights into evaluating the species composition and acetylcholinesterase (AChE) inhibitory activity of the culturable endophytic fungi of H. serrata from the regional at Jinggang Mountain in southeastern China. A total number of 885 fungal isolates distributed across 44 genera and 118 putative species were obtained from 1422 fragments of fine H. serrata roots, stems and leaves base on ITS-rDNA sequences BLAST analysis. The endophytic fungi were phylogenetically diverse and species-rich, with high rate of colonization and isolation. The assemble of endophytic fungi consisted mainly of Ascomycota (97.15%), followed by Basidiomycota (1.92%) and unknown fungal species (0.90%). Colletotrichum (64.29%), Phyllosticta (3.39%), Hypoxylon (2.81%), Xylaria (2.25%) and Nigrospora (2.04%) were the most abundant genera, whereas the remaining genera were infrequent groups. Although, roots yielded low abundance strains, the diverse and species-rich were both higher than that of stems and leaves. In addition, out of the 247 endophytic fungi strains determinated, 221 fungal extracts showed AChE inhibition activities in vitro. Among them, 22 endophytic fungi strains achieved high inhibitory activity (≥50%) on AChE which belongs to 13 genera and five incertae sedis strains. Four endophytic fungi designated as JS4 (Colletotrichum spp.), FL14 (Ascomycota spp.), FL9 (Sarcosomataceae spp.) and FL7 (Dothideomycetes spp.) were displayed highly active (≥80%) against AChE, which the inhibition effects were even more intense than the positive control. Our findings highlight that H. serrata grown in Jinggang Mountain harbors a rich and fascinating endophytic fungus community with potential AChE inhibitory activity, which could further broaden the natural acetylcholinesterase inhibitors resources used for Alzheimer's disease treatment.
蛇足石杉具有许多重要的药用特性，其药理潜力已得到证实。其中一些特性可能由其内生真菌介导。为了验证这一假设，在本研究中，我们首次对中国东南部井冈山地区蛇足石杉可培养内生真菌的物种组成和乙酰胆碱酯酶（AChE）抑制活性进行了评估。基于ITS-rDNA序列的BLAST分析，从1422段蛇足石杉的细根、茎和叶中获得了总共885株真菌分离物，它们分布在44个属和118个假定物种中。内生真菌在系统发育上具有多样性且物种丰富，定殖率和分离率较高。内生真菌的组合主要由子囊菌门（97.15%）组成，其次是担子菌门（1.92%）和未知真菌物种（0.90%）。炭疽菌属（64.29%）、叶点霉属（3.39%）、炭团菌属（2.81%）、炭角菌属（2.25%）和黑孢霉属（2.04%）是最丰富的属，而其余的属则是不常见的类群。虽然根产生的菌株丰度较低，但其多样性和物种丰富度均高于茎和叶。此外，在测定的247株内生真菌菌株中，有221株真菌提取物在体外显示出AChE抑制活性。其中，22株内生真菌菌株对AChE具有高抑制活性（≥50%），它们属于13个属和5个分类地位不确定的菌株。四种内生真菌，分别命名为JS4（炭疽菌属物种）、FL14（子囊菌门物种）、FL9（肉座菌科物种）和FL7（座囊菌纲物种），对AChE表现出高活性（≥80%），其抑制效果甚至比阳性对照更强。我们的研究结果表明，生长在井冈山的蛇足石杉拥有丰富且迷人的具有潜在AChE抑制活性的内生真菌群落，这可能进一步拓宽用于治疗阿尔茨海默病的天然乙酰胆碱酯酶抑制剂资源。
